التفاصيل البيبلوغرافية
| العنوان: |
Nutrient and metabolite gradients in mammalian cell hollow fiber bioreactors |
| المؤلفون: |
Piret, J. M., Devens, D. A., Cooney, C. L. |
| المصدر: |
The Canadian Journal of Chemical Engineering ; volume 69, issue 2, page 421-428 ; ISSN 0008-4034 1939-019X |
| بيانات النشر: |
Wiley |
| سنة النشر: |
1991 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
This work has investigated high density CRL‐1606 hybridoma cell culture in the shell‐side of ultrafiltration hollow fiber bioreactors. Oxygen depletion was measured in the recycle stream, and has been identified as the critical, axial scale‐limiting factor in these reactors. The composition of the extracapillary space has been studied by rapidly freezing these reactors in liquid nitrogen to recover samples of the shell‐side environment. The accumulation of inhibitory metabolites should have only a marginal impact on growth and product formation of this hybridoma cell line. Oxygen depletion is expected to be the major limiting factor for both the axial and radial scale‐up of hollow fiber bioreactors. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1002/cjce.5450690204 |
| الإتاحة: |
https://doi.org/10.1002/cjce.5450690204Test |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.2EBC0CD4 |
| قاعدة البيانات: |
BASE |
