دورية أكاديمية
Adipose Mesenchymal Extracellular Vesicles as Alpha-1-Antitrypsin Physiological Delivery Systems for Lung Regeneration
| العنوان: | Adipose Mesenchymal Extracellular Vesicles as Alpha-1-Antitrypsin Physiological Delivery Systems for Lung Regeneration |
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| المؤلفون: | Bari E, Ferrarotti I, Di Silvestre D, Grisoli P, Barzon V, Balderacchi A, Torre ML, Rossi R, MAURI, PAOLO, Corsico AG, Perteghella S |
| المساهمون: | Bari, E, Ferrarotti, I, Di Silvestre, D, Grisoli, P, Barzon, V, Balderacchi, A, Torre, Ml, Rossi, R, Mauri, Paolo, Corsico, Ag, Perteghella, S |
| سنة النشر: | 2019 |
| المجموعة: | Università degli Studi del Piemonte Orientale: CINECA IRIS |
| الوصف: | Accumulating evidence shows that Mesenchymal Stem/Stromal Cells (MSCs) exert their therapeutic effects by the release of secretome, made of both soluble proteins and nano/microstructured extracellular vesicles (EVs). In this work, for the first time, we proved by a proteomic investigation that adipose-derived (AD)-MSC-secretome contains alpha-1-antitrypsin (AAT), the main elastase inhibitor in the lung, 72 other proteins involved in protease/antiprotease balance, and 46 proteins involved in the response to bacteria. By secretome fractionation, we proved that AAT is present both in the soluble fraction of secretome and aggregated and/or adsorbed on the surface of EVs, that can act as natural carriers promoting AAT in vivo stability and activity. To modulate secretome composition, AD-MSCs were cultured in different stimulating conditions, such as serum starvation or chemicals (IL-1β and/or dexamethasone) and the expression of the gene encoding for AAT was increased. By testing in vitro the anti-elastase activity of MSC-secretome, a dose-dependent effect was observed; chemical stimulation of AD-MSCs did not increase their secretome anti-elastase activity. Finally, MSC-secretome showed anti-bacterial activity on Gram-negative bacteria, especially for Klebsiella pneumoniae. These preliminary results, in addition to the already demonstrated immunomodulation, pave the way for the use of MSC-secretome in the treatment of AAT-deficiency lung diseases. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/31450843; info:eu-repo/semantics/altIdentifier/wos/WOS:000489103800024; volume:23; issue:8; journal:CELLS; http://hdl.handle.net/11579/133243Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85077049525 |
| DOI: | 10.3390/cells8090965 |
| الإتاحة: | https://doi.org/10.3390/cells8090965Test http://hdl.handle.net/11579/133243Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.2DAEE660 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/cells8090965 |
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