التفاصيل البيبلوغرافية
| العنوان: |
Persistent Reptarenavirus and Hartmanivirus Infection in Cultured Boid Cells |
| المؤلفون: |
Lintala, Annika, Szirovicza, Leonora, Kipar, Anja, Hetzel, Udo, Hepojoki, Jussi |
| المساهمون: |
Department of Virology, University of Helsinki, Medicum, Viral Zoonosis Research Unit, Faculty of Veterinary Medicine, Helsinki One Health (HOH) |
| بيانات النشر: |
ASM Press |
| سنة النشر: |
2023 |
| المجموعة: |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
| مصطلحات موضوعية: |
arenavirus, cell culture, coinfection, persistence, LYMPHOCYTIC CHORIOMENINGITIS VIRUS, INCLUSION-BODY DISEASE, ARENAVIRUSES, SNAKES, REPLICATION, INHIBITION, 11832 Microbiology and virology |
| الوصف: |
Mammarenaviruses cause a persistent infection in their natural rodent and bat hosts. Reptarenaviruses cause boid inclusion body disease (BIBD) in constrictor snakes, but it is unclear whether snakes are the natural host of these viruses. Mammarenaviruses establish a persistent infection in their rodent and bat hosts, and the evidence suggests that reptarenaviruses and hartmaniviruses found in captive snakes act similarly. In snakes, reptarenaviruses cause boid inclusion body disease (BIBD), which is often associated with secondary infections. Snakes with BIBD usually carry more than a single pair of reptarenavirus S and L segments and occasionally demonstrate hartmanivirus coinfection. Here, we reported the generation of cell lines persistently infected with a single or two reptarenavirus(es) and a cell line with persistent reptarenavirus-hartmanivirus coinfection. By RT-PCR we demonstrated that the amount of viral RNA within the persistently infected cells remains at levels similar to those observed following initial infection. Using antibodies against the glycoproteins (GPs) and nucleoprotein (NP) of reptarenaviruses, we studied the levels of viral protein in cells passaged 10 times after the original inoculation and observed that the expression of GPs declines dramatically during persistent infection, unlike the expression of NP. Immunofluorescence (IF) staining served to demonstrate differences in the distribution of NP within the persistently infected compared to freshly infected cells. IF staining of cells inoculated with the viruses secreted from the persistently infected cell lines produced similar NP staining compared to cells infected with a traditionally passaged virus, suggesting that the altered NP expression pattern of persistently infected cells does not relate to changes in the virus. The cell cultures described herein can serve as tools for studying the coinfection and superinfection interplay between reptarenaviruses and studying the BIBD pathogenesis mechanisms. IMPORTANCE Mammarenaviruses ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
The study was supported by the Academy of Finland (J.H.; grants 308613, 314119, and 335762).; Lintala , A , Szirovicza , L , Kipar , A , Hetzel , U & Hepojoki , J 2022 , ' Persistent Reptarenavirus and Hartmanivirus Infection in Cultured Boid Cells ' , Microbiology Spectrum , vol. 10 , no. 4 , e0158522 . https://doi.org/10.1128/spectrum.01585-22Test; ORCID: /0000-0001-5699-214X/work/127502173; ORCID: /0000-0003-1528-044X/work/127508895; a9a06d42-a1ed-4cef-bffc-d977c59228b2; http://hdl.handle.net/10138/353714Test; 000823148800004 |
| الإتاحة: |
http://hdl.handle.net/10138/353714Test |
| حقوق: |
cc_by ; openAccess ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.2D101C40 |
| قاعدة البيانات: |
BASE |
