دورية أكاديمية
Impact of ceftiofur Injection on gut microbiota and Escherichia coli resistance in pigs
| العنوان: | Impact of ceftiofur Injection on gut microbiota and Escherichia coli resistance in pigs |
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| المؤلفون: | Fleury, M. A., Mourand, G., Jouy, E., Touzain, F., Le Devendec, L., de Boisseson, C., Eono, F., Cariolet, R., Guerin, Alizée, Le Goff, O., Blanquet-Diot, S., Alric, M., Kempf, I. |
| المساهمون: | Laboratoire de Ploufragan - Plouzané, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université de Brest (UBO), Université d'Auvergne - Clermont-Ferrand I (UdA), Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| المصدر: | ISSN: 0066-4804. |
| بيانات النشر: | HAL CCSD American Society for Microbiology |
| سنة النشر: | 2015 |
| المجموعة: | Anses: HAL (Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail) |
| مصطلحات موضوعية: | [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Resistance to extended-spectrum cephalosporins (ESCs) is an important health concern. Here, we studied the impact of the administration of a long-acting form of ceftiofur on the pig gut microbiota and ESC resistance in Escherichia coli . Pigs were orally inoculated with an ESC-resistant E. coli M63 strain harboring a conjugative plasmid carrying a gene conferring resistance, blaCTX-M-1. On the same day, they were given or not a unique injection of ceftiofur. Fecal microbiota were studied using quantitative PCR analysis of the main bacterial groups and quantification of short-chain fatty acids. E. coli and ESC-resistant E. coli were determined by culture methods, and the ESC-resistant E. coli isolates were characterized. The copies of the blaCTX-M-1 gene were quantified. After ceftiofur injection, the main change in gut microbiota was the significant but transitory decrease in the E. coli population. Acetate and butyrate levels were significantly lower in the treated group. In all inoculated groups, E. coli M63 persisted in most pigs, and the blaCTX-M-1 gene was transferred to other E. coli . Culture and PCR results showed that the ceftiofurtreated group shed significantly more resistant strains 1 and 3 days after ESC injection. Thereafter, on most dates, there were no differences between the groups, but notably, one pig in the nontreated group regularly excreted very high numbers of ESC-resistant E. coli , probably leading to a higher contamination level in its pen. In conclusion, the use of ESCs, and also the presence of high-shedding animals, are important features in the spread of ESC resistance. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-02637864; https://hal.inrae.fr/hal-02637864Test; https://hal.inrae.fr/hal-02637864/documentTest; https://hal.inrae.fr/hal-02637864/file/2015%20-%20Publi%2037%20-%20Guerin_1.pdfTest; PRODINRA: 341250; WOS: 000364343900011 |
| DOI: | 10.1128/AAC.00177-15 |
| الإتاحة: | https://doi.org/10.1128/AAC.00177-15Test https://hal.inrae.fr/hal-02637864Test https://hal.inrae.fr/hal-02637864/documentTest https://hal.inrae.fr/hal-02637864/file/2015%20-%20Publi%2037%20-%20Guerin_1.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.2CB4703F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/AAC.00177-15 |
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