التفاصيل البيبلوغرافية
| العنوان: |
The replication initiation protein Sld2 regulates helicase assembly. |
| المساهمون: |
Bruck, Irina (authoraut), Kaplan, Daniel L (authoraut) |
| سنة النشر: |
2014 |
| المجموعة: |
Florida State University: DigiNole Commons |
| مصطلحات موضوعية: |
Cell Cycle Proteins/genetics, Cell Cycle Proteins/metabolism, DNA Replication/physiology, DNA, Fungal/biosynthesis, Fungal/genetics, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Minichromosome Maintenance Complex Component 7/genetics, Minichromosome Maintenance Complex Component 7/metabolism, Minichromosome Maintenance Proteins/genetics, Minichromosome Maintenance Proteins/metabolism, Mutation, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, Protein Binding/physiology, S Phase/physiology, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism |
| الوصف: |
Assembly of the Cdc45-Mcm2-7-GINS (CMG) replicative helicase complex must be regulated to ensure that DNA unwinding is coupled with DNA synthesis. Sld2 is required for the initiation of DNA replication in budding yeast. We identified a mutant of Sld2, Sld2-m1,4, that is specifically defective in Mcm2-7 binding. When this sld2-m1,4 mutant is expressed, cells exhibit severe inhibition of DNA replication. Furthermore, the CMG complex assembles prematurely in G1 in mutant cells, but not wild-type cells. These data suggest that Sld2 binding to Mcm2-7 is essential to block the inappropriate formation of a CMG helicase complex in G1. We also study a mutant of Sld2 that is defective in binding DNA, sld2-DNA, and find that sld2-DNA cells exhibit no GINS-Mcm2-7 interaction. These data suggest that Sld2 association with DNA is required for CMG assembly in S phase. ; Keywords: CDK (Cyclin-dependent Kinase), Cell Cycle, DNA Helicase, DNA Replication, DNA-binding Protein ; Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900945Test. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
1 online resource; computer |
| اللغة: |
English |
| العلاقة: |
The Journal of biological chemistry--1083-351X--1083-351X; fsu:523882; (IID) FSU_pmch_24307213; (DOI) 10.1074/jbc.M113.532085; (PMCID) PMC3900945; (RID) 24307213; (EID) 24307213; (PII) M113.532085; https://diginole.lib.fsu.edu/islandora/object/fsu%3A523882/datastream/TN/view/replication%20initiation%20protein%20Sld2%20regulates%20helicase%20assembly.jpgTest |
| الإتاحة: |
https://diginole.lib.fsu.edu/islandora/object/fsu%3A523882/datastream/TN/view/replication%20initiation%20protein%20Sld2%20regulates%20helicase%20assembly.jpgTest |
| رقم الانضمام: |
edsbas.2C529F06 |
| قاعدة البيانات: |
BASE |
