دورية أكاديمية
Low expression of glycoprotein subunit 130 in ejaculated spermatozoa from asthenozoospermic men
| العنوان: | Low expression of glycoprotein subunit 130 in ejaculated spermatozoa from asthenozoospermic men |
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| المؤلفون: | Cai, Zhi-Ming, Gui, Yao-Ting, Guo, Xin, Yu, Jing, Guo, Lian-Dian, Zhang, Li-Bing, Wang, He, Yu, Jie |
| المساهمون: | Gui, YT (reprint author), Peking Univ, Shenzhen Hosp, Ctr Reprod Med, Lab Male Reprod Med, Shenzhen 518036, Peoples R China., Peking Univ, Shenzhen Hosp, Ctr Reprod Med, Lab Male Reprod Med, Shenzhen 518036, Peoples R China. |
| المصدر: | PubMed ; SCI |
| بيانات النشر: | 男科学杂志 |
| سنة النشر: | 2006 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | interleukin-6, human spermatozoa, motility, SPERM-MOTILITY, SEMINAL PLASMA, GENE-EXPRESSION, SERTOLI-CELLS, INFERTILE MEN, FERTILE MEN, RECEPTOR, RNA, SPERMATOGENESIS |
| الوصف: | Previous studies showed that interleukin-6 (IL-6) was expressed in human Leydig and Sertoli cells and that it inhibited sperm motility. The aim of this study was to compare the expression of IL-6, IL-6R, and GP130 in ejaculated spermatozoa between normozoospermic and asthenozoospermic men. Human spermatozoa in the semen were purified by Percoll gradient technique to separate the seminal plasma and other round cells. RT-PCR, immunocytochemistry, and Western blot were used to detect the expression of IL-6, IL-6R, and GP130 in spermatozoa. With RTPCR, only GP130 mRNA but not IL-6 and IL-6R mRNA was expressed in human ejaculated spermatozoa. The expression of GP130 mRNA was significantly lower in asthenozoospermic men than in normozoospermic men. The protein expression of GP130 was further confirmed by both immunocytochemistry and Western blot. Again, GP130 protein levels were significantly lower in asthenozoospermic men than in normozoospermic men. The results suggested that the decreased expression of GP130 in ejaculated spermatozoa could be associated with low sperm motility in asthenozoospermic men. ; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000240608200005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Test ; Andrology ; SCI(E) ; PubMed ; 8 ; ARTICLE ; 5 ; 645-652 ; 27 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 0196-3635 |
| العلاقة: | JOURNAL OF ANDROLOGY.2006,27,(5),645-652.; 674804; http://hdl.handle.net/20.500.11897/198860Test; WOS:000240608200005 |
| DOI: | 10.2164/jandrol.106.000562 |
| الإتاحة: | https://doi.org/20.500.11897/198860Test https://doi.org/10.2164/jandrol.106.000562Test https://hdl.handle.net/20.500.11897/198860Test |
| رقم الانضمام: | edsbas.2C516DF8 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 01963635 |
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| DOI: | 10.2164/jandrol.106.000562 |