دورية أكاديمية
Role of miR-146a in the Regulation of Inflammation in an In Vitro Model of Graves' Orbitopathy
| العنوان: | Role of miR-146a in the Regulation of Inflammation in an In Vitro Model of Graves' Orbitopathy |
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| المساهمون: | College of Medicine, Dept. of Ophthalmology, Sun Young Jang, Min Kyung Chae, Joon H. Lee, Eun Jig Lee, Jin Sook Yoon, Yoon, Jin Sook, Lee, Eun Jig |
| بيانات النشر: | Association For Research In Vision And Ophthalmology (Arvo) United States |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Blotting, Western, Cells, Cultured, Enzyme-Linked Immunosorbent Assay, Female, Fibroblasts/metabolism, Fibroblasts/pathology, Gene Expression Regulation, Graves Ophthalmopathy/genetics, Graves Ophthalmopathy/metabolism, Graves Ophthalmopathy/pathology, Humans, Inflammation/genetics, Inflammation/metabolism, Inflammation/pathology, Interleukin-6/metabolism, Male, MicroRNAs/biosynthesis, MicroRNAs/genetics, Microarray Analysis, RNA/genetics, Real-Time Polymerase Chain Reaction, miR-146a, inflammation, Graves�� orbitopathy |
| الوصف: | PURPOSE: To investigate the role of microRNA 146a (miR-146a) in the regulation of inflammation in an in vitro model of Graves' orbitopathy (GO). METHODS: The level of miR-146a expression in orbital adipose tissue was compared between GO and non-GO by quantitative real-time PCR (qPCR). The effects of interleukin 1棺 (IL-1棺) on miR-146a expression were analyzed in orbital fibroblasts by qPCR. To investigate the molecular mechanism underlying IL-1棺-induced miR-146a expression, the effects of inhibitors of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-觀B), mitogen-activated protein kinase/extracellular signal-regulated kinases (MEK)-1/2, c-Jun N-terminal kinases (JNK)-1/2, p38 MAP kinase, and phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) were analyzed. The effects of miR-146a mimics and inhibitors on IL-1棺-induced IL-6 release were examined by ELISA and Western blotting. RESULTS: The level of miR-146a expression was significantly higher in GO orbital adipose tissue than in non-GO (P = 0.032). Interleukin 1棺 induced a time- and concentration-dependent increase in miR-146a expression. Interleukin 1棺 (10 ng/mL, 16 hours) induced an approximately 17.5-fold increase in miR-146 expression. The increase in miR-146a expression by IL-1棺 was significantly inhibited by NF-觀B, JNK-1/2, and PI3K inhibitors (1.94? 짹 ?0.25, 5.28? 짹 ?0.34 and 9.73? 짹 ?2.32-fold, respectively, P < 0.05 compared with IL-1棺-induced miR-146 expression, independent t-test). Interleukin 1棺-induced IL-6 protein production was further decreased by miR-146a mimics, but not by inhibitors of miR-146a. CONCLUSIONS: MicroRNA 146a was upregulated by inflammatory stress in orbital fibroblasts. Our results indicated that miR-146a had a positive effect on the anti-inflammatory process. MicroRNA 146a may play a role in the regulation of inflammation in orbital fibroblasts, and may participate in the pathogenesis of GO. ; restriction |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0146-0404 1552-5783 |
| العلاقة: | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE; J01187; OAK-2016-05111; https://ir.ymlib.yonsei.ac.kr/handle/22282913/151927Test; http://iovs.arvojournals.org/article.aspx?articleid=2543210Test; T201603451; INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.57(10) : 4027-4034, 2016 |
| DOI: | 10.1167/iovs.16-19213 |
| الإتاحة: | https://doi.org/10.1167/iovs.16-19213Test https://ir.ymlib.yonsei.ac.kr/handle/22282913/151927Test http://iovs.arvojournals.org/article.aspx?articleid=2543210Test |
| حقوق: | CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/krTest/ |
| رقم الانضمام: | edsbas.2B4A0993 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 01460404 15525783 |
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| DOI: | 10.1167/iovs.16-19213 |