التفاصيل البيبلوغرافية
| العنوان: |
Epigenetic Manipulation to Trigger Production of Guaiane-Type Sesquiterpenes from a Marine-Derived Spiromastix sp. Fungus with Antineuroinflammatory Effects |
| المؤلفون: |
Xiang Guo (223678), Qinyu Meng (11015308), Siwen Niu (1816708), Jie Liu (15128), Xingchen Guo (11015311), Zhaolun Sun (11015314), Dong Liu (115204), Yucheng Gu (1670197), Jian Huang (7250), Aili Fan (2236705), Wenhan Lin (471132) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Molecular Biology, Immunology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Epigenetic Manipulation, SAHA, sediment-derived Spiromastix sp, TNF -α dose-dependently, terpene-related biosynthetic gene c., anti-neuroinflammatory activity, suberoylanilide hydroxamic acid, Antineuroinflammatory Effects Epige., Trigger Production, configurational assignments, spectroscopic techniques, COX -2, ECD, BV -2 cells, transcription levels, mRNA levels, LPS-induced BV -2, Guaiane-Type Sesquiterpenes, SAR analyses, multifunctional agent, BV -2 microglial cells, spiromaterpenes A-I, chemical conversion, LPS-induced NF -κB translocation, guaiane-type sesquiterpenoids |
| الوصف: |
Epigenetic manipulation of a deep-sea sediment-derived Spiromastix sp. fungus using suberoylanilide hydroxamic acid (SAHA) induction resulted in the activation of a terpene-related biosynthetic gene cluster, and nine new guaiane-type sesquiterpenes, spiromaterpenes A-I ( 1 – 9 ), were isolated. Their structures were determined using various spectroscopic techniques, in association with the modified Mosher’s method, computed electronic circular dichroism (ECD) spectra, and chemical conversion for configurational assignments. Compounds 4 – 6 exhibited significant effects against the NO production on lipopolysaccharide (LPS)-induced microglia cells BV2, and the preliminary SAR analyses demonstrated that a 2( R ),11-diol unit is favorable. The most active 5 abolished LPS-induced NF-κB translocation from the cytosol to the nucleus in BV-2 microglial cells, accompanied by the marked reduction of the transcription levels of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α dose-dependently in both LPS-induced BV-2 and BV-2 cells, as well as the protein and mRNA levels of iNOS and COX-2. This study complements the gap in knowledge regarding the anti-neuroinflammatory activity of guaiane-type sesquiterpenoids at the cellular level and suggests that 5 is promising for further optimization as a multifunctional agent for antineuroinflammation. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Epigenetic_Manipulation_to_Trigger_Production_of_Guaiane-Type_Sesquiterpenes_from_a_Marine-Derived_i_Spiromastix_i_sp_Fungus_with_Antineuroinflammatory_Effects/14832268Test |
| DOI: |
10.1021/acs.jnatprod.1c00293.s001 |
| الإتاحة: |
https://doi.org/10.1021/acs.jnatprod.1c00293.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.2A06DB9 |
| قاعدة البيانات: |
BASE |
