دورية أكاديمية
Scalable production of tissue-like vascularized liver organoids from human PSCs ; ENEngelskEnglishScalable production of tissue-like vascularized liver organoids from human PSCs
| العنوان: | Scalable production of tissue-like vascularized liver organoids from human PSCs ; ENEngelskEnglishScalable production of tissue-like vascularized liver organoids from human PSCs |
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| المؤلفون: | Harrison, Sean Philip, Siller, Richard, Tanaka, Yoshiaki, Chollet Dugarte, Maria Eugenia, de la Morena-Barrio, María Eugenia, Xiang, Yangfei, Patterson, Benjamin, Andersen, Elisabeth, Bravo-Pérez, Carlos, Kempf, Henning, Nordhus, Kathrine Sivertsen, Lunov, Oleg, Dejneka, Alexandr, Mowinckel, Marie-Christine, Stavik, Benedicte, Sandset, Per Morten, Melum, Espen, Baumgarten, Saphira Felicitas, Bonanini, Flavio, Kurek, Dorota, Mathapati, Santosh Sadashiv, Almaas, Runar, Sharma, Kulbhushan, Wilson, Steven Ray Haakon, Skottvoll, Frøydis Sved, Boger, Ida Caroline Sneis, Bogen, Inger Lise, Nyman, Tuula Anneli, Wu, Jun Jie, Bezrouk, Ales, Cizkova, Dana, Corral, Javier, Mokry, Jaroslav, Zweigerdt, Robert, Park, In-Hyun, Sullivan, Gareth John |
| المصدر: | 1226-3613. |
| سنة النشر: | 2023 |
| المجموعة: | Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
| الوصف: | The lack of physiological parity between 2D cell culture and in vivo culture has led to the development of more organotypic models, such as organoids. Organoid models have been developed for a number of tissues, including the liver. Current organoid protocols are characterized by a reliance on extracellular matrices (ECMs), patterning in 2D culture, costly growth factors and a lack of cellular diversity, structure, and organization. Current hepatic organoid models are generally simplistic and composed of hepatocytes or cholangiocytes, rendering them less physiologically relevant compared to native tissue. We have developed an approach that does not require 2D patterning, is ECM independent, and employs small molecules to mimic embryonic liver development that produces large quantities of liver-like organoids. Using single-cell RNA sequencing and immunofluorescence, we demonstrate a liver-like cellular repertoire, a higher order cellular complexity, presenting with vascular luminal structures, and a population of resident macrophages: Kupffer cells. The organoids exhibit key liver functions, including drug metabolism, serum protein production, urea synthesis and coagulation factor production, with preserved post-translational modifications such as N-glycosylation and functionality. The organoids can be transplanted and maintained long term in mice producing human albumin. The organoids exhibit a complex cellular repertoire reflective of the organ and have de novo vascularization and liver-like function. These characteristics are a prerequisite for many applications from cellular therapy, tissue engineering, drug toxicity assessment, and disease modeling to basic developmental biology. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Harrison, Sean Philip Siller, Richard Tanaka, Yoshiaki Chollet Dugarte, Maria Eugenia de la Morena-Barrio, María Eugenia Xiang, Yangfei Patterson, Benjamin Andersen, Elisabeth Bravo-Pérez, Carlos Kempf, Henning Nordhus, Kathrine Sivertsen Lunov, Oleg Dejneka, Alexandr Mowinckel, Marie-Christine Stavik, Benedicte Sandset, Per Morten Melum, Espen Baumgarten, Saphira Felicitas Bonanini, Flavio Kurek, Dorota Mathapati, Santosh Sadashiv Almaas, Runar Sharma, Kulbhushan Wilson, Steven Ray Haakon Skottvoll, Frøydis Sved Boger, Ida Caroline Sneis Bogen, Inger Lise Nyman, Tuula Anneli Wu, Jun Jie Bezrouk, Ales Cizkova, Dana Corral, Javier Mokry, Jaroslav Zweigerdt, Robert Park, In-Hyun Sullivan, Gareth John . Scalable production of tissue-like vascularized liver organoids from human PSCs. Experimental and Molecular Medicine. 2023, 55, 2005-2024; http://hdl.handle.net/10852/107135Test; 2184397; info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Experimental and Molecular Medicine&rft.volume=55&rft.spage=2005&rft.date=2023; Experimental and Molecular Medicine; 55; 2005; 2024; https://doi.org/10.1038/s12276-023-01074-1Test |
| DOI: | 10.1038/s12276-023-01074-1 |
| الإتاحة: | https://doi.org/10.1038/s12276-023-01074-1Test http://hdl.handle.net/10852/107135Test |
| حقوق: | Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.2A05C869 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s12276-023-01074-1 |
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