دورية أكاديمية
The role of cysteine residues in the sulphate transporter, SHST1:Construction of a functional cysteine-less transporter
| العنوان: | The role of cysteine residues in the sulphate transporter, SHST1:Construction of a functional cysteine-less transporter |
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| المؤلفون: | Howitt, Susan |
| المصدر: | Biochimica et Biophysica Acta: International journal of Biochemistry and Biophysics |
| بيانات النشر: | Elsevier |
| المجموعة: | Australian National University: ANU Digital Collections |
| مصطلحات موضوعية: | Keywords: alanine, amino acid, carrier protein, carrier protein shst1, cysteine, leucine, sulfate, unclassified drug, article, controlled study, mutant, nonhuman, nucleotide sequence, priority journal, protein function, protein structure, wild type, yeast, Amino Ac Congenital chloride diarrhoea, Diastrophic dysplasia, Pendred syndrome, Site-directed mutagenesis, Sulphate transporter |
| الوصف: | We investigated the role of cysteine residues in the sulphate transporter, SHST1, with the aim of generating a functional cysteine-less variant. SHST1 contains five cysteine residues and none was essential for function. However, replacement of C421 resulted in a reduction in transport activity. Sulphate transport by C205 mutants was dependent on the size of the residue at this position. Alanine at position 205 resulted in a complete loss of function whereas leucine resulted in a 3-fold increase in sulphate transport relative to wild type SHST1. C205 is located in a putative intracellular loop and our results suggest that this loop may be important for sulphate transport. By replacing C205 with leucine and the other four cysteine residues with alanine, we constructed a cysteine-less variant of SHST1 that has transport characteristics indistinguishable from wild type. This construct will be useful for further structure and function studies of SHST1. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0006-3002 |
| العلاقة: | http://hdl.handle.net/1885/83121Test; https://openresearch-repository.anu.edu.au/bitstream/1885/83121/5/MigratedxPub11330_2005.pdf.jpgTest; https://openresearch-repository.anu.edu.au/bitstream/1885/83121/7/01_Howitt_The_role_of_cysteine_residues_2005.pdf.jpgTest |
| DOI: | 10.1016/j.bbamem.2005.01.002 |
| الإتاحة: | https://doi.org/10.1016/j.bbamem.2005.01.002Test http://hdl.handle.net/1885/83121Test https://openresearch-repository.anu.edu.au/bitstream/1885/83121/5/MigratedxPub11330_2005.pdf.jpgTest https://openresearch-repository.anu.edu.au/bitstream/1885/83121/7/01_Howitt_The_role_of_cysteine_residues_2005.pdf.jpgTest |
| رقم الانضمام: | edsbas.297360B7 |
| قاعدة البيانات: | BASE |
| تدمد: | 00063002 |
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| DOI: | 10.1016/j.bbamem.2005.01.002 |