التفاصيل البيبلوغرافية
| العنوان: |
Investigating how the Tumour Microenvironment Influences the Alternative Splicing of Ribosomal Protein S24 and the Splicing Machinery Involved |
| المؤلفون: |
Goodbrand, Jenna |
| المساهمون: |
Uniacke, James |
| بيانات النشر: |
University of Guelph |
| سنة النشر: |
2023 |
| المجموعة: |
University of Guelph: DSpace digital archive |
| مصطلحات موضوعية: |
Tumour microenvironment, Splicing, Ribosomal Protein S24, Diagnostic markers, Cancer |
| الوصف: |
Hypoxia-induced alternative splicing has been coined the 11th hallmark of cancer. Previous research has revealed that an alternative splicing event occurs in ribosomal protein S24 (RPS24) in response to hypoxia, with further induction in three-dimensional tumour models, called spheroids, enabling enhanced survival under hypoxic conditions. Our findings indicate that increased cell-to-cell contact and apoptosis in spheroids play a significant role in the upregulation of the RPS24L to RPS24S isoform ratio. Further exploration of the splicing machinery unveiled the involvement of serine and arginine-rich splicing factor 1 (SRSF1). Knockdown or inhibition of SRSF1 resulted in decreased expression of both RPS24 isoforms. By investigating the induction of RPS24 alternative splicing, our objective is to contribute to the exploration of RPS24 splice variants as potential diagnostic markers for early-stage cancer detection. Ultimately, elucidating the splicing factors involved may shed light on their potential as therapeutic targets for cancer patients. |
| نوع الوثيقة: |
thesis |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
https://hdl.handle.net/10214/27747Test |
| الإتاحة: |
https://hdl.handle.net/10214/27747Test |
| حقوق: |
Attribution-NoDerivatives 4.0 International ; http://creativecommons.org/licenses/by-nd/4.0Test/ |
| رقم الانضمام: |
edsbas.28DCC10E |
| قاعدة البيانات: |
BASE |
