التفاصيل البيبلوغرافية
| العنوان: |
A Practical Guide to Rodent Islet Isolation and Assessment |
| المؤلفون: |
Jeffrey D. Carter, Stacey B. Dula, Kathryn L. Corbin, Runpei Wu, Craig S. Nunemaker |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/18/70/Biol_Proced_Online_2009_Dec_3_11_3-31.tar.gz |
| المجموعة: |
CiteSeerX |
| مصطلحات موضوعية: |
Key words, islets of Langerhans, pancreatic islets, beta-cell, beta cells, cytokines, transplantation, calcium, insulin, apoptosis, necrosis, isolation, islet isolation, collagenase, rodent, mouse, rat, islet |
| الوصف: |
Pancreatic islets of Langerhans secrete hormones that are vital to the regulation of blood glucose and are, therefore, a key focus of diabetes research. Purifying viable and functional islets from the pancreas for study is an intricate process. This review highlights the key elements involved with mouse and rat islet isolation, including choices of collagenase, the collagenase digestion process, purification of islets using a density gradient, and islet culture conditions. In addition, this paper reviews commonly used techniques for assessing islet viability and function, including visual assessment, fluorescent markers of cell death, glucose-stimulated insulin secretion, and intracellular calcium measurements. A detailed protocol is also included that describes a common method for rodent islet isolation that our laboratory uses to obtain viable and functional mouse islets for in vitro study of islet function, beta-cell physiology, and in vivo rodent islet transplantation. The purpose of this review is to serve as a resource and foundation for successfully procuring and purifying high-quality islets for research purposes. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/zip |
| اللغة: |
English |
| العلاقة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.349.2509Test |
| حقوق: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.2795281A |
| قاعدة البيانات: |
BASE |
