دورية أكاديمية
The Occurrence and resistance of Shigella flexneri CECT4804 to acid stress: Impact of acid stress in vitro and in vivo
| العنوان: | The Occurrence and resistance of Shigella flexneri CECT4804 to acid stress: Impact of acid stress in vitro and in vivo |
|---|---|
| المؤلفون: | Taieb, Ines, Ellafi, Ali, Ben Younes, Sonia, Feriani, Anouar, Bakhrouf, Amina, Elmzoughi, Ridha, Serrano, Juan Alfonso Ayala, Jabeur, Chedia |
| سنة النشر: | 2023 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| الوصف: | The ability to maintain intra-cellular pH is crucial for many microbes mainly the enterobacteria to survive in diverse environments, particularly those that undergo fluctuations in pH. In this context, the growth and survival of S. flexneri at different acid pH values were evaluated to explain the response strategies involved in the adaptation of Shigella flexneri CECT4804 in acid stress conditions. Furthermore, the capacity of this strain to produce slime on Congo Red Agar, biofilm formation on polystyrene plate and hydrophobicity are reported. In addition, the modification of the membrane fatty acids profiles has been studied. Moreover, an infection with the stressed strain was realized on rats in rates and examined for their toxicity in intestine tissue. The obtained results show that the strain survival is strongly influenced by acidity. Indeed, the stressed and unstressed strains became biofilm positive after acid stress. A significant increase in the hydrophobicity percentage compared to the values found under normal conditions, is also noticed. For the membrane fatty acids analysis, the acidity induces several modifications in the membrane composition. After the infection, the gravest lesion was registered in the intestine of rats administered with the bacteria stressed at the lowest pH. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1828-1427 |
| العلاقة: | Publisher's version; http://dx.doi.org/10.12834/VetIt.2529.18140.2Test; Sí; Veterinaria Italiana 59 (2023); http://hdl.handle.net/10261/357338Test |
| DOI: | 10.12834/VetIt.2529.18140.2 |
| الإتاحة: | https://doi.org/10.12834/VetIt.2529.18140.2Test http://hdl.handle.net/10261/357338Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.276B1C11 |
| قاعدة البيانات: | BASE |
| تدمد: | 18281427 |
|---|---|
| DOI: | 10.12834/VetIt.2529.18140.2 |