التفاصيل البيبلوغرافية
| العنوان: |
Connecting GCN5’s centromeric SAGA to the mitotic tension-sensing checkpoint |
| المؤلفون: |
Petty, Emily L, Evpak, Masha, Pillus, Lorraine |
| المساهمون: |
Yamashita, Yukiko |
| المصدر: |
Molecular Biology of the Cell, vol 29, iss 18 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2018 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Biochemistry and Cell Biology, Biological Sciences, Human Genome, Genetics, 1.1 Normal biological development and functioning, Underpinning research, Generic health relevance, Centromere, Chromatids, Chromosomal Proteins, Non-Histone, Chromosome Segregation, Chromosomes, DNA-Binding Proteins, Histone Acetyltransferases, Humans, Kinetochores, M Phase Cell Cycle Checkpoints, Microtubules, Mitosis, Nuclear Proteins, Protein Phosphatase 2, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Medical and Health Sciences, Developmental Biology |
| الوقت: |
2201 - 2212 |
| الوصف: |
Multiple interdependent mechanisms ensure faithful segregation of chromosomes during cell division. Among these, the spindle assembly checkpoint monitors attachment of spindle microtubules to the centromere of each chromosome, whereas the tension-sensing checkpoint monitors the opposing forces between sister chromatid centromeres for proper biorientation. We report here a new function for the deeply conserved Gcn5 acetyltransferase in the centromeric localization of Rts1, a key player in the tension-sensing checkpoint. Rts1 is a regulatory component of protein phopshatase 2A, a near universal phosphatase complex, which is recruited to centromeres by the Shugoshin (Sgo) checkpoint component under low-tension conditions to maintain sister chromatid cohesion. We report that loss of Gcn5 disrupts centromeric localization of Rts1. Increased RTS1 dosage robustly suppresses gcn5∆ cell cycle and chromosome segregation defects, including restoration of Rts1 to centromeres. Sgo1's Rts1-binding function also plays a key role in RTS1 dosage suppression of gcn5∆ phenotypes. Notably, we have identified residues of the centromere histone H3 variant Cse4 that function in these chromosome segregation-related roles of RTS1. Together, these findings expand the understanding of the mechanistic roles of Gcn5 and Cse4 in chromosome segregation. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt7rq9c71t; https://escholarship.org/uc/item/7rq9c71tTest |
| الإتاحة: |
https://escholarship.org/uc/item/7rq9c71tTest |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.23C4E079 |
| قاعدة البيانات: |
BASE |
