دورية أكاديمية
A report on the international tranglutaminase autoantibody workshop for celiac disease
| العنوان: | A report on the international tranglutaminase autoantibody workshop for celiac disease |
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| المؤلفون: | LI M, YU L, TAKI I, MIAO D, MURRAY YA, REWERS MJ, HOFFENBERG EJ, AGARDH D, MUELLER P, STERN M, BONIFACIO E, LIU E., TIBERTI, Claudio, BONAMICO, Margherita |
| المساهمون: | Li, M, Yu, L, Tiberti, Claudio, Bonamico, Margherita, Taki, I, Miao, D, Murray, Ya, Rewers, Mj, Hoffenberg, Ej, Agardh, D, Mueller, P, Stern, M, Bonifacio, E, Liu, E. |
| بيانات النشر: | Elsevier Science Incorporated / NY Journals:Madison Square Station, PO Box 882:New York, NY 10159:(212)633-3730, EMAIL: usinfo-f@elsevier.com, INTERNET: http://www.elsevier.comTest, Fax: (212)633-3680 |
| سنة النشر: | 2009 |
| المجموعة: | Sapienza Università di Roma: CINECA IRIS |
| الوصف: | OBJECTIVES: Measurement of transglutaminase autoantibodies (TGAA) is considered to be the most efficient single serologic test for celiac disease (CD) by the American Gastroenterological Association Institute. We hypothesized that a large international collaborative effort toward improving and standardizing TGAA measurement is both feasible and necessary. The primary aim of this workshop is to compare TGAA assays among various research and clinical laboratories to examine assay concordance and improve (and eventually standardize) the TGAA assay. METHODS: A total of 20 laboratories (5 commercial laboratories, 15 research and clinical laboratories) participated that included enzyme-linked immunosorbent assay (ELISA) and radiobinding assays. A total of 150 serum samples were distributed to each laboratory, with each laboratory receiving an equal aliquot that was coded and blinded, composed of 100 healthy control sera and 50 CD sera. RESULTS: Laboratory sensitivity ranged from 69% to 93% and specificity ranged from 96% to 100%. By receiver operator characteristic analysis, the area under the curve (C index) ranged from 0.9488 to 0.9904. When analyzing for linear correlation, r-squared was as high as 0.8882 but as low as 0.4244 for the celiac samples between different laboratories performing ELISA. CONCLUSIONS: This transglutaminase autoantibody workshop allows for larger-scale international participation for the purposes of improving and eventually standardizing the TGAA assay with subsequent workshops. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/19098864; info:eu-repo/semantics/altIdentifier/wos/WOS:000262265800026; volume:104; firstpage:154; lastpage:163; numberofpages:10; journal:THE AMERICAN JOURNAL OF GASTROENTEROLOGY; http://hdl.handle.net/11573/98339Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-60749134816; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000262265800026&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396aTest; http://www.scopus.com/inward/record.url?eid=2-s2.0-60749134816&partnerID=65&md5=b0f977fb3177b057785346f94d1da2acTest |
| DOI: | 10.1038/ajg.2008.8 |
| الإتاحة: | https://doi.org/10.1038/ajg.2008.8Test http://hdl.handle.net/11573/98339Test http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000262265800026&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396aTest http://www.scopus.com/inward/record.url?eid=2-s2.0-60749134816&partnerID=65&md5=b0f977fb3177b057785346f94d1da2acTest |
| رقم الانضمام: | edsbas.22B9C5F0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/ajg.2008.8 |
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