دورية أكاديمية
Vitamin D3 down-regulates intracellular Toll-like receptor 9 expression and Toll-like receptor 9-induced IL-6 production in human monocytes
| العنوان: | Vitamin D3 down-regulates intracellular Toll-like receptor 9 expression and Toll-like receptor 9-induced IL-6 production in human monocytes |
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| المؤلفون: | Dickie, Laura J., Church, Leigh D., Coulthard, Lydia R., Mathews, Rebeccah J., Emery, Paul, McDermott, Michael F. |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2010 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | BASIC SCIENCE |
| الوصف: | Objective. To determine whether vitamin D 3 modulates monocytic expression of intracellular Toll-like receptors (TLRs) 3, 7 and 9. Methods. Human monocytes were isolated from peripheral blood and cultured with 100 nM vitamin D 3 for 24, 48 and 72 h. Expression of CD14 and TLR2, TLR3, TLR4, TLR7 and TLR9 were examined by flow cytometry. Monocytes exposed to vitamin D 3 for 48 h were then stimulated with a TLR9 agonist for a further 24 h. The level of IL-6 secretion was measured by ELISA. Results. CD14 was up-regulated, whereas TLR2, TLR4 and TLR9 expression was down-regulated by vitamin D 3 exposure in a time-dependent manner. TLR3 expression was unaffected by vitamin D 3 and there was no measurable expression of TLR7 on the monocytes. TLR9-induced IL-6 production was impaired in monocytes treated with vitamin D 3 compared with untreated cells. Conclusion. The intracellular TLRs are differentially regulated by vitamin D 3 , with TLR9 being down-regulated by vitamin D 3 exposure whereas TLR3 was unaffected. This decreased TLR9 expression in monocytes had a downstream functional effect as these cells subsequently secreted less IL-6 in response to TLR9 challenge. This may have significant biological relevance and may be a factor in the association of vitamin D deficiency with susceptibility to autoimmune disease. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://rheumatology.oxfordjournals.org/cgi/content/short/49/8/1466Test; http://dx.doi.org/10.1093/rheumatology/keq124Test |
| DOI: | 10.1093/rheumatology/keq124 |
| الإتاحة: | https://doi.org/10.1093/rheumatology/keq124Test http://rheumatology.oxfordjournals.org/cgi/content/short/49/8/1466Test |
| حقوق: | Copyright (C) 2010, British Society for Rheumatology |
| رقم الانضمام: | edsbas.229AFEE6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/rheumatology/keq124 |
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