دورية أكاديمية
Validated extended multiplexed LC-MS/MS assay for the quantification of adagrasib and sotorasib in human plasma, together with four additional SMIs
| العنوان: | Validated extended multiplexed LC-MS/MS assay for the quantification of adagrasib and sotorasib in human plasma, together with four additional SMIs |
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| المؤلفون: | Kruithof, Paul D, de Beer, Yvo M, Gulikers, Judith L, Stolk, Leo M L, Hendriks, Lizza E L, Croes, Sander, van Geel, Robin M J M |
| المصدر: | Kruithof , P D , de Beer , Y M , Gulikers , J L , Stolk , L M L , Hendriks , L E L , Croes , S & van Geel , R M J M 2023 , ' Validated extended multiplexed LC-MS/MS assay for the quantification of adagrasib and sotorasib in human plasma, together with four additional SMIs ' , Journal of chromatography. B, Analytical technologies in the biomedical and life sciences , vol. 1231 , no. 1 , 123918 . https://doi.org/10.1016/j.jchromb.2023.123918Test |
| سنة النشر: | 2023 |
| المجموعة: | Maastricht University Research Publications |
| مصطلحات موضوعية: | Adagrasib, Non-Small Cell Lung Cancer, Pharmacokinetics, Sotorasib, Validation |
| الوصف: | Recently, two small molecular inhibitors (SMIs) -adagrasib and sotorasib- have been introduced for targeting Kirsten rat sarcoma (KRAS) p.G12C mutations in patients with non-small cell lung cancer (NSCLC). In order to support pharmacokinetic research as well as clinical decision making, we developed and validated a simple and accurate liquid chromatography-tandem mass spectrometry method for the multiplexed quantification of adagrasib and sotorasib. This assay was co-validated with the quantification for brigatinib, lorlatinib, pralsetinib and selpercatinib. Methanol was used for single-step protein precipitation. Chromatographic separation was performed using an Acquity® HSS C18 UPLC column, with an elution gradient of ammonium formate 0.1 % / in water and acetonitrile. In K2-EDTA plasma, adagrasib was found to be stable for at least seven days at room temperature and 4 °C, and at least 3 months at -80 °C. Sotorasib was found to be stable for at least three days at room temperature, seven days at 4 °C and at least 3 months at -80 °C. The method was validated over a linear range of 80-4000 ng/mL for adagrasib and 25-2500 ng/mL for sotorasib. The assay is therefore well-equipped for determining plasma concentrations in clinical practice. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://cris.maastrichtuniversity.nl/en/publications/b53c21bc-3f8f-44e2-9d83-94cfc8af2b91Test |
| DOI: | 10.1016/j.jchromb.2023.123918 |
| الإتاحة: | https://doi.org/10.1016/j.jchromb.2023.123918Test https://cris.maastrichtuniversity.nl/en/publications/b53c21bc-3f8f-44e2-9d83-94cfc8af2b91Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.224412FD |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jchromb.2023.123918 |
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