التفاصيل البيبلوغرافية
| العنوان: |
Haemophilus parasuis (Glaesserella parasuis) as a Potential Driver of Molecular Mimicry and Inflammation in Rheumatoid Arthritis |
| المؤلفون: |
Di Sante, Gabriele, Gremese, Elisa, Tolusso, Barbara, Cattani, Paola, Di Mario, Clara, Marchetti, Simona, Alivernini, Stefano, Tredicine, Maria, Petricca, Luca, Palucci, Ivana, Camponeschi, Chiara, Aragon, Virginia, Gambotto, Andrea, Ria, Francesco, Ferraccioli, Gianfranco |
| المساهمون: |
Fondazione Italiana Sclerosi Multipla, Università Cattolica del Sacro Cuore |
| المصدر: |
Frontiers in Medicine ; volume 8 ; ISSN 2296-858X |
| بيانات النشر: |
Frontiers Media SA |
| سنة النشر: |
2021 |
| المجموعة: |
Frontiers (Publisher - via CrossRef) |
| الوصف: |
Background: Haemophilus parasuis ( Hps ; now Glaesserella parasuis ) is an infectious agent that causes severe arthritis in swines and shares sequence similarity with residues 261–273 of collagen type 2 (Coll 261−273 ), a possible autoantigen in rheumatoid arthritis (RA). Objectives/methods: We tested the presence of Hps sequencing 16S ribosomal RNA in crevicular fluid, synovial fluids, and tissues in patients with arthritis (RA and other peripheral arthritides) and in healthy controls. Moreover, we examined the cross-recognition of Hps by Coll 261−273 -specific T cells in HLA-DRB1 * 04 pos RA patients, by T-cell receptor (TCR) beta chain spectratyping and T-cell phenotyping. Results: Hps DNA was present in 57.4% of the tooth crevicular fluids of RA patients and in 31.6% of controls. Anti- Hps IgM and IgG titers were detectable and correlated with disease duration and the age of the patients. Peripheral blood mononuclear cells (PBMCs) were stimulated with Hps virulence-associated trimeric autotransporter peptide (VtaA10 755−766 ), homologous to human Coll 261−273 or co-cultured with live Hps . In both conditions, the expanded TCR repertoire overlapped with Coll 261−273 and led to the production of IL-17. Discussion: We show that the DNA of an infectious agent ( Hps ), not previously described as pathogen in humans, is present in most patients with RA and that an Hps peptide is able to activate T cells specific for Coll 261−273 , likely inducing or maintaining a molecular mimicry mechanism. Conclusion: The cross-reactivity between VtaA10 755−766 of a non-human infectious agent and human Coll 261−273 suggests an involvement in the pathogenesis of RA. This mechanism appears emphasized in predisposed individuals, such as patients with shared epitope. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| DOI: |
10.3389/fmed.2021.671018 |
| DOI: |
10.3389/fmed.2021.671018/full |
| الإتاحة: |
https://doi.org/10.3389/fmed.2021.671018Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.20DE63E |
| قاعدة البيانات: |
BASE |
