التفاصيل البيبلوغرافية
| العنوان: |
Table_3_Single-Cell Transcriptomics and In Situ Morphological Analyses Reveal Microglia Heterogeneity Across the Nigrostriatal Pathway.docx |
| المؤلفون: |
Oihane Uriarte Huarte, Dimitrios Kyriakis, Tony Heurtaux, Yolanda Pires-Afonso, Kamil Grzyb, Rashi Halder, Manuel Buttini, Alexander Skupin, Michel Mittelbronn, Alessandro Michelucci |
| سنة النشر: |
2021 |
| المجموعة: |
Frontiers: Figshare |
| مصطلحات موضوعية: |
Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, microglia, nigrostriatal pathway, single-cell transcriptomics, cellular heterogeneity, immune alerted, cell morphology, Parkinson’s disease |
| الوصف: |
Microglia are the resident immune effector cells of the central nervous system (CNS) rapidly reacting to various pathological stimuli to maintain CNS homeostasis. However, microglial reactions in the CNS may also worsen neurological disorders. Hence, the phenotypic analysis of microglia in healthy tissue may identify specific poised subsets ultimately supporting or harming the neuronal network. This is all the more important for the understanding of CNS disorders exhibiting regional-specific and cellular pathological hallmarks, such as many neurodegenerative disorders, including Parkinson’s disease (PD). In this context, we aimed to address the heterogeneity of microglial cells in susceptible brain regions for PD, such as the nigrostriatal pathway. Here, we combined single-cell RNA-sequencing with immunofluorescence analyses of the murine nigrostriatal pathway, the most affected brain region in PD. We uncovered a microglia subset, mainly present in the midbrain, displaying an intrinsic transcriptional immune alerted signature sharing features of inflammation-induced microglia. Further, an in situ morphological screening of inferred cellular diversity showed a decreased microglia complexity in the midbrain when compared to striatum. Our study provides a resource for the identification of specific microglia phenotypes within the nigrostriatal pathway, which may be relevant in PD. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/dataset/Table_3_Single-Cell_Transcriptomics_and_In_Situ_Morphological_Analyses_Reveal_Microglia_Heterogeneity_Across_the_Nigrostriatal_Pathway_docx/14331848Test |
| DOI: |
10.3389/fimmu.2021.639613.s004 |
| الإتاحة: |
https://doi.org/10.3389/fimmu.2021.639613.s004Test
https://figshare.com/articles/dataset/Table_3_Single-Cell_Transcriptomics_and_In_Situ_Morphological_Analyses_Reveal_Microglia_Heterogeneity_Across_the_Nigrostriatal_Pathway_docx/14331848Test |
| حقوق: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.1FD94A12 |
| قاعدة البيانات: |
BASE |
