دورية أكاديمية
Cross-talk between motor neurons and myotubes via endogenously secreted neural and muscular growth factors
| العنوان: | Cross-talk between motor neurons and myotubes via endogenously secreted neural and muscular growth factors |
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| المؤلفون: | Saini, J, Faroni, A, Reid, AJ, Mouly, V, Butler-Browne, G, Lightfoot, AP, McPhee, JS, Degens, H, Al-Shanti, N |
| بيانات النشر: | Wiley |
| سنة النشر: | 2021 |
| المجموعة: | Liverpool John Moores University: LJMU Research Online |
| مصطلحات موضوعية: | QH301 Biology, QP Physiology, RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry, RC1200 Sports Medicine |
| الوصف: | Neuromuscular junction (NMJ) research is vital to advance the understanding of neuromuscular patho-physiology and development of novel therapies for diseases associated with NM dysfunction. In vivo, the micro-environment surrounding the NMJ has a significant impact on NMJ formation and maintenance via neurotrophic and differentiation factors that are secreted as a result of cross-talk between muscle fibers and motor neurons. Recently we showed the formation of functional NMJs in vitro in a co-culture of immortalized human myoblasts and motor neurons from rat-embryo spinal-cord explants, using a culture medium free from serum and neurotrophic or growth factors. The aim of this study was to assess how functional NMJs were established in this co-culture devoid of exogenous neural growth factors. To investigate this, an ELISA-based microarray was used to compare the composition of soluble endogenously secreted growth factors in this co-culture with an a-neural muscle culture. The levels of seven neurotrophic factors brain-derived neurotrophic factor (BDNF), glial-cell-line-derived neurotrophic factor (GDNF), insulin-like growth factor-binding protein-3 (IGFBP-3), insulin-like growth factor-1 (IGF-1), neurotrophin-3 (NT-3), neurotrophin-4 (NT-4), and vascular endothelial growth factor (VEGF) were higher (p < 0.05) in the supernatant of NMJ culture compared to those in the supernatant of the a-neural muscle culture. This indicates that the cross-talk between muscle and motor neurons promotes the secretion of soluble growth factors contributing to the local microenvironment thereby providing a favourable regenerative niche for NMJs formation and maturation. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | https://researchonline.ljmu.ac.uk/id/eprint/16801/1/Cross-talk%20between%20motor%20neurons%20and%20myotubes%20via%20endogenously%20secreted%20neural%20and%20muscular%20growth%20factors.pdfTest; Saini, J, Faroni, A, Reid, AJ, Mouly, V, Butler-Browne, G, Lightfoot, AP, McPhee, JS, Degens, H and Al-Shanti, N (2021) Cross-talk between motor neurons and myotubes via endogenously secreted neural and muscular growth factors. Physiological Reports, 9 (8). e14791. ISSN 2051-817X |
| DOI: | 10.14814/phy2.14791 |
| الإتاحة: | https://doi.org/10.14814/phy2.14791Test http://researchonline.ljmu.ac.uk/id/eprint/16801Test/ https://researchonline.ljmu.ac.uk/id/eprint/16801/1/Cross-talk%20between%20motor%20neurons%20and%20myotubes%20via%20endogenously%20secreted%20neural%20and%20muscular%20growth%20factors.pdfTest |
| حقوق: | cc_by |
| رقم الانضمام: | edsbas.1F90975D |
| قاعدة البيانات: | BASE |
| DOI: | 10.14814/phy2.14791 |
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