دورية أكاديمية
Transport of gold nanoparticles by vascular endothelium from different human tissues
| العنوان: | Transport of gold nanoparticles by vascular endothelium from different human tissues |
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| المساهمون: | Kaya, Mehmet (ORCID 0000-0001-8318-1350 & YÖK ID 10486), Gromnicova, Radka, Romero, Ignacio A., Williams, Phil, Satchell, Simon, Sharrack, Basil, Male, David, School of Medicine, Department of Physiology |
| المصدر: | PLOS One |
| بيانات النشر: | Public Library of Science International |
| سنة النشر: | 2016 |
| المجموعة: | Koç University Suna Kıraç Library’ Digital Collections |
| مصطلحات موضوعية: | Multidisciplinary sciences, Blood-brain-barrier, Cellular uptake, Lipid-bilayers, In-vitro, Surface-charge, Glycocalyx, Size, Cells, Penetration, Lectin |
| الوصف: | The selective entry of nanoparticles into target tissues is the key factor which determines their tissue distribution. Entry is primarily controlled by microvascular endothelial cells, which have tissue-specific properties. This study investigated the cellular properties involved in selective transport of gold nanoparticles (<5 nm) coated with PEG-amine/galactose in two different human vascular endothelia. Kidney endothelium (ciGENC) showed higher uptake of these nanoparticles than brain endothelium (hCMEC/D3), reflecting their biodistribution in vivo. Nanoparticle uptake and subcellular localisation was quantified by transmission electron microscopy. The rate of internalisation was approximately 4x higher in kidney endothelium than brain endothelium. Vesicular endocytosis was approximately 4x greater than cytosolic uptake in both cell types, and endocytosis was blocked by metabolic inhibition, whereas cytosolic uptake was energy-independent. The cellular basis for the different rates of internalisation was investigated. Morphologically, both endothelia had similar profiles of vesicles and cell volumes. However, the rate of endocytosis was higher in kidney endothelium. Moreover, the glycocalyces of the endothelia differed, as determined by lectin-binding, and partial removal of the glycocalyx reduced nanoparticle uptake by kidney endothelium, but not brain endothelium. This study identifies tissue-specific properties of vascular endothelium that affects their interaction with nanoparticles and rate of transport. ; Sheffield Teaching Hospitals Trust; Biotechnology and Biological Sciences Research Council |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | Publisher version; Koç University Institutional Repository; 10486; Gromnicova, R., et al. "Transport of Gold Nanoparticles by Vascular Endothelium from Different Human Tissues." PLOS One 11.8 (2016): 17.; http://dx.doi.org/10.1371/journal.pone.0161610Test; IR00830; WoS; Scopus; PubMed; BB/K009184/ 1; NA; http://libdigitalcollections.ku.edu.tr/cdm/ref/collection/IR/id/834Test |
| DOI: | 10.1371/journal.pone.0161610 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0161610Test http://libdigitalcollections.ku.edu.tr/cdm/ref/collection/IR/id/834Test |
| رقم الانضمام: | edsbas.1ECDCD3B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0161610 |