دورية أكاديمية
JAK-STAT-dependent contact between follicle cells and the oocyte controls Drosophila anterior-posterior polarity and germline development
| العنوان: | JAK-STAT-dependent contact between follicle cells and the oocyte controls Drosophila anterior-posterior polarity and germline development |
|---|---|
| المؤلفون: | Mallart, Charlotte, Netter, Sophie, Chalvet, Fabienne, Claret, Sandra, Guichet, Antoine, Montagne, Jacques, Pret, Anne-Marie, Malartre, Marianne |
| المساهمون: | Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut Jacques Monod (IJM (UMR_7592)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), We wish to thank Erika Bach, Jean-Paul Vincent, Anne Ephrussi, Vincent Mirouse, Véronique Brodu and Antoine Boivin for sharing fly stocks and Anne Ephrussi, Talila Volt, and Robert Edwin Ward for sharing antibodies. We are grateful to Fred Bernard for helpful discussions and Jean-René Huynh for valuable comments on the manuscript. We also thank Flybase, BDSC (Bloomington Drosophila Stock Center), VDRC (Vienna Drosophila Resource Center), DGGR (Kyoto Drosophila Stock Center), DSHB (Developmental Studies Hybridoma Bank) for resources and the Imagerie-Gif core facility supported by the Agence Nationale de la Recherche (ANR-11-EQPX-0029/Morphoscope, ANR-10-INBS-04/FranceBioImaging, ANR-11-IDEX-0003-02/Saclay Plant Sciences) for confocal imaging and the ImagoSeine core facility of the Institut Jacques Monod member of IBiSA and the France-BioImaging (ANR-10-INBS-04) infrastructure for EM experiments. This work was supported by a 3 year-PhD fellowship obtained from the “Ministère de l’Enseignement Supérieur et de la Recherche” for CM, by the Center National de Recherche Scientifique (CNRS), Paris-Saclay and Versailles St-Quentin-en-Yvelines Universities, by a grant from the “Ligue contre le cancer” for laboratory expenses (AMP), and by a grant from the “Fondation ARC pour la recherche sur le cancer” for a year of salary expenses for CM., ANR-11-EQPX-0029,MORPHOSCOPE 2,Imagerie et reconstruction multiéchelles de la morphogenèse. (Plateforme d'innovation technologique et méthodologique pour l'imagerie in vivo et la reconstruction des dynamiques multiéchelles de la morphogenèse)(2011), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), ANR-11-IDEX-0003,IPS,Idex Paris-Saclay(2011) |
| المصدر: | ISSN: 2041-1723. |
| بيانات النشر: | HAL CCSD Nature Publishing Group |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | MESH: Animals, MESH: Drosophila, MESH: Drosophila Proteins, MESH: Oocytes, MESH: Oogenesis, MESH: Germ Cells, MESH: Cell Polarity, MESH: Drosophila melanogaster, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; The number of embryonic primordial germ cells in Drosophila is determined by the quantity of germ plasm, whose assembly starts in the posterior region of the oocyte during oogenesis. Here, we report that extending JAK-STAT activity in the posterior somatic follicular epithelium leads to an excess of primordial germ cells in the future embryo. We show that JAK-STAT signaling is necessary for the differentiation of approximately 20 specialized follicle cells maintaining tight contact with the oocyte. These cells define, in the underlying posterior oocyte cortex, the anchoring of the germ cell determinant oskar mRNA. We reveal that the apical surface of these posterior anchoring cells extends long filopodia penetrating the oocyte. We identify two JAK-STAT targets in these cells that are each sufficient to extend the zone of contact with the oocyte, thereby leading to production of extra primordial germ cells. JAK-STAT signaling thus determines a fixed number of posterior anchoring cells required for anterior-posterior oocyte polarity and for the development of the future germline. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/38388656; hal-04595801; https://u-paris.hal.science/hal-04595801Test; PUBMED: 38388656; PUBMEDCENTRAL: PMC10883949 |
| DOI: | 10.1038/s41467-024-45963-z |
| الإتاحة: | https://doi.org/10.1038/s41467-024-45963-zTest https://u-paris.hal.science/hal-04595801Test |
| رقم الانضمام: | edsbas.1DB50E6D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-024-45963-z |
|---|