دورية أكاديمية
Patterns of within-host genetic diversity in SARS-CoV-2 ...
| العنوان: | Patterns of within-host genetic diversity in SARS-CoV-2 ... |
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| المؤلفون: | Tonkin-Hill, Gerry, Martincorena, Inigo, Amato, Roberto, Lawson, Andrew, Gerstung, Moritz, Johnston, Ian, Jackson, David, Park, Naomi, Lensing, Stefanie, Quail, Michael, Gonçalves, Sónia, Ariani, Cristina, Chapman, Michael Spencer, Hamilton, William, Meredith, Luke, Hall, Grant, Jahun, Aminu, Chaudhry, Yasmin, Hosmillo, Myra, Pinckert, Malte, Georgana, Iliana, Yakovleva, Anna, Caller, Laura, Caddy, Sarah, Feltwell, Theresa, Khokhar, Fahad, Houldcroft, Charlotte, Curran, Martin, Parmar, Surendra, Alderton, Alex, Nelson, Rachel, Harrison, Ewan, Sillitoe, John, Bentley, Stephen, Barrett, Jeffrey, Torok, Estee, Goodfellow, Ian, Langford, Cordelia, Kwiatkowski, Dominic, The COVID-19 Genomics UK COG-UK Consortium, Wellcome Sanger Institute COVID-19 Surveillance Team |
| بيانات النشر: | Cold Spring Harbor Laboratory |
| سنة النشر: | 2020 |
| المجموعة: | DataCite Metadata Store (German National Library of Science and Technology) |
| مصطلحات موضوعية: | The COVID-19 Genomics UK COG-UK Consortium, Wellcome Sanger Institute COVID-19 Surveillance Team |
| الوصف: | Monitoring the spread of SARS-CoV-2 and reconstructing transmission chains has become a major public health focus for many governments around the world. The modest mutation rate and rapid transmission of SARS-CoV-2 prevents the reconstruction of transmission chains from consensus genome sequences, but within-host genetic diversity could theoretically help identify close contacts. Here we describe the patterns of within-host diversity in 1,181 SARS-CoV-2 samples sequenced to high depth in duplicate. 95% of samples show within-host mutations at detectable allele frequencies. Analyses of the mutational spectra revealed strong strand asymmetries suggestive of damage or RNA editing of the plus strand, rather than replication errors, dominating the accumulation of mutations during the SARS-CoV-2 pandemic. Within and between host diversity show strong purifying selection, particularly against nonsense mutations. Recurrent within-host mutations, many of which coincide with known phylogenetic homoplasies, display a ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.17863/cam.91081 |
| الإتاحة: | https://doi.org/10.17863/cam.91081Test https://www.repository.cam.ac.uk/handle/1810/343655Test |
| حقوق: | open.access ; Creative Commons Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0/legalcodeTest ; cc-by-4.0 ; http://purl.org/coar/access_right/c_abf2Test |
| رقم الانضمام: | edsbas.1D416FF1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/cam.91081 |
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