التفاصيل البيبلوغرافية
| العنوان: |
Atlas of Musculoskeletal Stem Cells with the Soft and Hard Tissue Differentiation Architecture |
| المؤلفون: |
Yin, Zi, Lin, Junxin, Yan, Ruojin, Liu, Richun, Liu, Mengfei, Zhou, Bo, Zhou, Wenyan, An, Chengrui, Chen, Yangwu, Hu, Yejun, Fan, Chunmei, Zhao, Kun, Wu, Bingbing, Zou, Xiaohui, Zhang, Jin, El‐Hashash, Ahmed H., Chen, Xiao, Ouyang, Hongwei |
| المساهمون: |
National Natural Science Foundation of China |
| المصدر: |
Advanced Science ; volume 7, issue 23 ; ISSN 2198-3844 2198-3844 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2020 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Although being of utmost importance for human health and mobility, stem cell identity and hierarchical organization of musculoskeletal progenitors remain largely unexplored. Here, cells from E10.5, E12.5, and E15.5 murine limbs are analyzed by high throughput single‐cell RNA sequencing to illustrate the cellular architecture during limb development. Single‐cell transcriptional profiling demonstrates the identity and differentiation architecture of musculoskeletal stem cells (MSSC), soft and hard tissue progenitors through expression pattern of musculoskeletal markers (scleraxis [ Scx ], Hoxd13 , Sox9 , and Col1a1 ). This is confirmed by genetic in vivo lineage tracing. Moreover, single‐cell analyses of Scx knockout mice tissues illustrates that Scx regulates MSSC self‐renewal and proliferation potential. A high‐throughput and low‐cost multi‐tissues RNA sequencing strategy further provides evidence that musculoskeletal system tissues, including muscle, bone, meniscus, and cartilage, are all abnormally developed in Scx knockout mice. These results establish the presence of an indispensable limb Scx+Hoxd13+ MSSC population and their differentiation into soft tissue progenitors ( Scx+Col1a1+ ) and hard tissue progenitors ( Scx+Sox9+ ). Collectively, this study paves the way for systematically decoding the complex molecular mechanisms and cellular programs of musculoskeletal tissues morphogenesis in limb development and regeneration. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1002/advs.202000938 |
| الإتاحة: |
https://doi.org/10.1002/advs.202000938Test |
| حقوق: |
http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.1CE1C7D |
| قاعدة البيانات: |
BASE |
