دورية أكاديمية
Acceleration of pro-caspase-3 maturation and cell migration inhibition in human breast cancer cells by phytoconstituents of Rheum emodi rhizome extracts
| العنوان: | Acceleration of pro-caspase-3 maturation and cell migration inhibition in human breast cancer cells by phytoconstituents of Rheum emodi rhizome extracts |
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| المؤلفون: | Naveen Kumar, D. R., Cijo George, V., Suresh, P. K., Kumar, R. Ashok |
| سنة النشر: | 2013 |
| المجموعة: | Technische Universität Dortmund: Eldorado |
| مصطلحات موضوعية: | ddc:610, cancer-specific cytotoxicity, ER-negative breast cancer, aspase-3, anti-metastasis, HPLC, GC-MS |
| الوصف: | The aggressive nature of estrogen receptor (ER)-negative breast cancer subtype obligates for innovative targeted therapies. The present study aimed to investigate the phytoconstituents and specific anticancer activities of Rheum emodi rhizome, a known food source used locally to treat various ailments. Petroleum ether extracts (hot [PHR] and cold [PCR]) of R. emodi, exhibited significant free radical scavenging potentials through DPPH and reducing power assays, rendering them as good sources of antioxidants. The extracts, PHR and PCR had shown significant (P < 0.05) cancer-cell-specific cytotoxicity in the assayed cells (MDA-MB-231 [breast carcinoma] and WRL-68 [non-tumoral]) at 100 μg/ml, and 50 and 100 μg/ml concentrations respectively. Extracts also induced fervent apoptosis in ER-negative cells (MDA-MB-231) compared to ER-positive subtype (MCF-7), and found to involve CPP32/caspase-3 in its apoptosis induction mechanism. Moreover, extracts had an inevitable potential to inhibit the migration of metastatic breast cancer cells (MDA-MB-231)in vitro. Further, the active principles of extracts were identified through HPLC and GC-MS analysis to reveal major polyphenolics, 4,7-Dimethyl-(octahydro)indolo[4,3-fg]quinolin-10-one, 5-Oxo-isolongifolene, Valencene-2, and other quinone, quinoline and anthraquinone derivatives. The extracts are thus good candidates to target malignant ER-negative breast cancer, and the identified phytoconstituents could be eluted in further exploratory studies for use in dietary-based anti-breast cancer therapies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1611-2156 |
| العلاقة: | EXCLI Journal; Vol. 12, 2013; http://hdl.handle.net/2003/32931Test; http://dx.doi.org/10.17877/DE290R-7556Test |
| DOI: | 10.17877/DE290R-7556 |
| الإتاحة: | https://doi.org/10.17877/DE290R-7556Test http://hdl.handle.net/2003/32931Test |
| حقوق: | http://rightsstatements.org/page/InC/1.0Test/ |
| رقم الانضمام: | edsbas.1BAB14BC |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 16112156 |
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| DOI: | 10.17877/DE290R-7556 |