التفاصيل البيبلوغرافية
| العنوان: |
Expanding the toolbox for phycobiliprotein assembly: phycoerythrobilin biosynthesis in Synechocystis |
| المؤلفون: |
Heck, Steffen, Sommer, Frederik, Zehner, Susanne, Schroda, Michael, Gehringer, Michelle M., Frankenberg‐Dinkel, Nicole |
| المساهمون: |
Deutsche Forschungsgemeinschaft |
| المصدر: |
Physiologia Plantarum ; volume 176, issue 1 ; ISSN 0031-9317 1399-3054 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2023 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Phycobiliproteins (PBPs) play a vital role in light harvesting by cyanobacteria, which enables efficient utilization of photon energy for oxygenic photosynthesis. The PBPs carry phycobilins, open‐chain tetrapyrrole chromophores derived from heme. The structure and chromophore composition of PBPs is dependent on the organism's ecological niche. In cyanobacteria, these holo‐proteins typically form large, macromolecular antenna complexes called phycobilisomes (PBSs). The PBS of Synechocystis sp. PCC 6803 (hereafter Synechocystis ) consists of allophycocyanin (APC) and phycocyanin (PC), which exclusively harbor phycocyanobilin (PCB) as a chromophore. Investigations into heterologous PBP biosynthesis in E. coli have proven limiting with respect to PBP assembly and their functional characterization. Consequently, we wanted to engineer a platform for the investigation of heterologously produced PBPs, focusing on unusual, phycoerythrobilin (PEB)‐containing light‐harvesting proteins called phycoerythrins (PEs) in Synechocystis . As a first step, a gene encoding for the synthesis of the natural cyanobacterial chromophore, PEB, was introduced into Synechocystis . We provide spectroscopic evidence for heterologous PEB formation and show covalent attachment of PEB to the α‐subunit of PC, CpcA, by HPLC and LC–MS/MS analyses. Fluorescence microscopy and PBS isolation demonstrate a cellular dispersal of PBPs with modified phycobilin content. However, these modifications have minor effects on physiological responses, as demonstrated by growth rates, oxygen evolution, nutrient accumulation, and PBP content analyses. As a result, Synechocystis demonstrates the capacity to efficiently manage PEB biosynthesis and therefore reflects a promising platform for both biochemical and physiological investigations of foreign and unusual PEs. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1111/ppl.14137 |
| الإتاحة: |
https://doi.org/10.1111/ppl.14137Test |
| حقوق: |
http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.1BA2FFA4 |
| قاعدة البيانات: |
BASE |
