دورية أكاديمية
A novel MARV glycoprotein-specific antibody with potentials of broad-spectrum neutralization to filovirus
| العنوان: | A novel MARV glycoprotein-specific antibody with potentials of broad-spectrum neutralization to filovirus |
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| المؤلفون: | Zhang, Yuting, Zhang, Min, Wu, Haiyan, Wang, Xinwei, Zheng, Hang, Feng, Junjuan, Wang, Jing, Luo, Longlong, Xiao, He, Qiao, Chunxia, Li, Xinying, Zheng, Yuanqiang, Huang, Weijin, Wang, Youchun, Wang, Yi, Shi, Yanchun, Feng, Jiannan, Chen, Guojiang |
| المساهمون: | National Natural Science Foundation of China |
| المصدر: | eLife ; volume 12 ; ISSN 2050-084X |
| بيانات النشر: | eLife Sciences Publications, Ltd |
| سنة النشر: | 2024 |
| المجموعة: | eLife (E-Journal - via CrossRef) |
| الوصف: | Marburg virus (MARV) is one of the filovirus species that cause deadly hemorrhagic fever in humans, with mortality rates up to 90%. Neutralizing antibodies represent ideal candidates to prevent or treat virus disease. However, no antibody has been approved for MARV treatment to date. In this study, we identified a novel human antibody named AF-03 that targeted MARV glycoprotein (GP). AF-03 possessed a high binding affinity to MARV GP and showed neutralizing and protective activities against the pseudotyped MARV in vitro and in vivo. Epitope identification, including molecular docking and experiment-based analysis of mutated species, revealed that AF-03 recognized the Niemann-Pick C1 (NPC1) binding domain within GP1. Interestingly, we found the neutralizing activity of AF-03 to pseudotyped Ebola viruses (EBOV, SUDV, and BDBV) harboring cleaved GP instead of full-length GP. Furthermore, NPC2-fused AF-03 exhibited neutralizing activity to several filovirus species and EBOV mutants via binding to CI-MPR. In conclusion, this work demonstrates that AF-03 represents a promising therapeutic cargo for filovirus-caused disease. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.7554/elife.91181.3 |
| الإتاحة: | https://doi.org/10.7554/elife.91181.3Test https://cdn.elifesciences.org/articles/91181/elife-91181-v1.pdfTest https://cdn.elifesciences.org/articles/91181/elife-91181-v1.xmlTest https://elifesciences.org/articles/91181Test |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ ; http://creativecommons.org/licenses/by/4.0Test/ ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.1B846EB6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.7554/elife.91181.3 |
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