دورية أكاديمية
Organ-specific metabolic profiles of the liver and kidney during brain death and afterwards during normothermic machine perfusion of the kidney
| العنوان: | Organ-specific metabolic profiles of the liver and kidney during brain death and afterwards during normothermic machine perfusion of the kidney |
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| المؤلفون: | van Erp, Anne C, Qi, Haiyun, Jespersen, Nichlas R, Hjortbak, Marie V, Ottens, Petra J, Wiersema-Buist, J, Nørregaard, Rikke, Pedersen, Michael, Laustsen, Christoffer, Leuvenink, Henri G D, Jespersen, Bente |
| المصدر: | van Erp , A C , Qi , H , Jespersen , N R , Hjortbak , M V , Ottens , P J , Wiersema-Buist , J , Nørregaard , R , Pedersen , M , Laustsen , C , Leuvenink , H G D & Jespersen , B 2020 , ' Organ-specific metabolic profiles of the liver and kidney during brain death and afterwards during normothermic machine perfusion of the kidney ' , American Journal of Transplantation , vol. 20 , no. 9 , pp. 2425-2436 . https://doi.org/10.1111/ajt.15885Test |
| سنة النشر: | 2020 |
| المجموعة: | Aarhus University: Research |
| مصطلحات موضوعية: | animal models, basic (laboratory) research/science, donors and donation: donation after brain death (DBD), graft survival, kidney (allograft) function/dysfunction, kidney transplantation/nephrology, liver allograft function/dysfunction, liver transplantation/hepatology, organ procurement and allocation, translational research/science, basic (laboratory) research, REDOX STATE, kidney transplantation, hepatology, GRAFT, TRANSPLANTATION, donors and donation, dysfunction, nephrology, GLUCONEOGENESIS, GLUCOSE, MAGNETIC-RESONANCE, translational research, HYPERPOLARIZED C-13, RENAL VIABILITY, DONOR, KETONE-BODY RATIO, donation after brain death (DBD), science, kidney (allograft) function |
| الوصف: | We investigated metabolic changes during brain death (BD) using hyperpolarized magnetic resonance (MR) spectroscopy and ex vivo graft glucose metabolism during normothermic isolated perfused kidney (IPK) machine perfusion. BD was induced in mechanically ventilated rats by inflation of an epidurally placed catheter; sham-operated rats served as controls. Hyperpolarized [1- 13 C]pyruvate MR spectroscopy was performed to quantify pyruvate metabolism in the liver and kidneys at 3 time points during BD, preceded by injecting hyperpolarized[1- 13 C]pyruvate. Following BD, glucose oxidation was measured using tritium-labeled glucose (d-6-3H-glucose) during IPK reperfusion. Quantitative polymerase chain reaction and biochemistry were performed on tissue/plasma. Immediately following BD induction, lactate increased in both organs (liver: eµ d 0.21, 95% confidence interval [CI] [−0.27, −0.15]; kidney: eµ d 0.26, 95% CI [−0.40, −0.12]. After 4 hours of BD, alanine production decreased in the kidney (eµ d 0.14, 95% CI [0.03, 0.25], P <.05). Hepatic lactate and alanine profiles were significantly different throughout the experiment between groups (P <.01). During IPK perfusion, renal glucose oxidation was reduced following BD vs sham animals (eµ d 0.012, 95% CI [0.004, 0.03], P <.001). No differences in enzyme activities were found. Renal gene expression of lactate-transporter MCT4 increased following BD (P <.01). In conclusion, metabolic processes during BD can be visualized in vivo using hyperpolarized magnetic resonance imaging and with glucose oxidation during ex vivo renal machine perfusion. These techniques can detect differences in the metabolic profiles of the liver and kidney following BD. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://pure.au.dk/portal/en/publications/1526f829-fbd0-4481-b36e-02e07b014033Test |
| DOI: | 10.1111/ajt.15885 |
| الإتاحة: | https://doi.org/10.1111/ajt.15885Test https://pure.au.dk/portal/en/publications/1526f829-fbd0-4481-b36e-02e07b014033Test https://pure.au.dk/ws/files/220037037/ajt.15885.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.18701F14 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/ajt.15885 |
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