دورية أكاديمية
Exploring polyamine metabolism of Alternaria alternata to target new substances to control the fungal infection.
| العنوان: | Exploring polyamine metabolism of Alternaria alternata to target new substances to control the fungal infection. |
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| المؤلفون: | Estiarte, Núria, Crespo Sempere, Ana, Marín Sillué, Sònia, Sanchís Almenar, Vicente, Ramos Girona, Antonio J. |
| بيانات النشر: | Elsevier Ltd. |
| سنة النشر: | 2017 |
| المجموعة: | Universitat de Lleida: Repositori Obert UdL |
| مصطلحات موضوعية: | micotoxina, Alternaria, Alternariol |
| الوصف: | Polyamines are essential for all living organisms as they are involved in several vital cell functions. The biosynthetic pathway of polyamines and its regulation is well established and, in this sense, the ornithine descarboxylase (ODC) enzyme acts as one of the controlling factors of the entire pathway. In this work we assessed the inhibition of the ODC with D, l-α-difluoromethylornithine (DFMO) on Alternaria alternata and we observed that fungal growth and mycotoxin production were reduced. This inhibition was not completely restored by the addition of exogenous putrescine. Actually, increasing concentrations of putrescine on the growth media negatively affected mycotoxin production, which was corroborated by the downregulation of pksJ and altR, both genes involved in mycotoxin biosynthesis. We also studied the polyamine metabolism of A. alternata with the goal of finding new targets that compromise its growth and its mycotoxin production capacity. In this sense, we tested two different polyamine analogs, AMXT-2455 and AMXT-3016, and we observed that they partially controlled A. alternata's viability in vitro and in vivo using tomato plants. Finding strategies to design new fungicide substances is becoming a matter of interest as resistance problems are emerging. ; We thank Dr. Mark Burns, Aminex Therapeutics Inc. (USA), for kindly supply the polyamine analogs. We are also grateful to the Catalonian Government (XaRTA-Reference Network on Food Technology) for their financial support. N. Estiarte thanks the Secretaria de Universitats i Recerca del Departament d’Economia i Coneixement of the Generalitat de Catalunya for the predoctoral grant (FI-DGR 2013). |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0740-0020 |
| العلاقة: | Versió postprint del document publicat a: https://doi.org/10.1016/j.fm.2017.02.001Test; Food Microbiology, 2017, vol. 65, p. 193-204; http://hdl.handle.net/10459.1/59787Test; https://doi.org/10.1016/j.fm.2017.02.001Test |
| DOI: | 10.1016/j.fm.2017.02.001 |
| الإتاحة: | https://doi.org/10.1016/j.fm.2017.02.001Test http://hdl.handle.net/10459.1/59787Test |
| حقوق: | cc-by-nc-nd (c) Elsevier, 2017 ; http://creativecommons.org/licenses/by-nc-nd/4.0Test ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.16D99CEE |
| قاعدة البيانات: | BASE |
| تدمد: | 07400020 |
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| DOI: | 10.1016/j.fm.2017.02.001 |