دورية أكاديمية
Engineering sulfotransferases to modify heparan sulfate
| العنوان: | Engineering sulfotransferases to modify heparan sulfate |
|---|---|
| المؤلفون: | Xu, Ding, Moon, Andrea F, Song, Danyin, Pedersen, Lars C, Liu, Jian |
| المصدر: | Nature Chemical Biology, 4(3) |
| سنة النشر: | 2008 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | heparan sulfate D-glucosaminyl 3-O-sulfotransferase, Carbohydrate Conformation, Protein Engineering, Binding Sites, Feasibility Studies, Sulfotransferases, Protein Isoforms, Heparitin Sulfate, Models, Molecular, Substrate Specificity, Mutagenesis, Site-Directed |
| الوصف: | The biosynthesis of heparan sulfate (HS), an essential glycan in many organisms, involves an array of specialized sulfotransferases. Here, we present a study aimed at engineering the substrate specificity of different HS 3-O-sulfotransferase isoforms. Based on the crystal structures, we identified a pair of amino acid residues responsible for selecting the substrates. Mutations at these residues altered the substrate specificities. Our results demonstrated the feasibility of tailoring the specificity of sulfotransferases to modify HS with desired functions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.17615/rs67-bm97Test; https://cdr.lib.unc.edu/downloads/nv935979m?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/nv935979mTest |
| DOI: | 10.17615/rs67-bm97 |
| الإتاحة: | https://doi.org/10.17615/rs67-bm97Test https://cdr.lib.unc.edu/downloads/nv935979m?file=thumbnailTest https://cdr.lib.unc.edu/downloads/nv935979mTest |
| حقوق: | http://rightsstatements.org/vocab/InC/1.0Test/ |
| رقم الانضمام: | edsbas.16342104 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/rs67-bm97 |
|---|