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Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept

التفاصيل البيبلوغرافية
العنوان: Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept
المؤلفون: Gómez‑González, Emilio, Barriga‑Rivera, Alejandro, Fernández‑Muñoz, Beatriz, Navas‑García, José Manuel, Fernández‑Lizaranzu, Isabel, Munoz‑González, Francisco Javier, Parrilla‑Giráldez, Rubén, Requena‑Lancharro, Desiree, Gil‑Gamboa, Pedro, Rosell‑Valle, Crsitina, Gómez‑González, Carmen, Mayorga‑Buiza, Maria José, Martín‑López, María, Muñoz, Olga, Gómez Martín, Juan Carlos, Relimpio López, María Isabel, Aceituno‑Castro, Jesús, Perales‑Esteve, Manuel A., Puppo‑Moreno, Antonio, García Cózar, Francisco José, Olvera Collantes, Lucía, Gómez‑Díaz, Raquel, de los Santos‑Trigo, Silvia, Huguet‑Carrasco, Montserrat, Rey, Manuel, Gómez, Emilia, Sánchez Pernaute, Rosario, Padillo‑Ruiz, Javier, Márquez‑Rivas, Javier
المساهمون: Biomedicina, Biotecnología y Salud Pública
المصدر: Scientific Reports, Vol. 12, Núm. 1
بيانات النشر: NATURE RESEARCH
سنة النشر: 2022
المجموعة: RODIN - Repositorio de Objetos de Docencia e Investigación de la Universidad de Cádiz
الوصف: Effective testing is essential to control the coronavirus disease 2019 (COVID-19) transmission. Here we report a-proof-of-concept study on hyperspectral image analysis in the visible and near-infrared range for primary screening at the point-of-care of SARS-CoV-2. We apply spectral feature descriptors, partial least square-discriminant analysis, and artificial intelligence to extract information from optical diffuse reflectance measurements from 5 mu L fluid samples at pixel, droplet, and patient levels. We discern preparations of engineered lentiviral particles pseudotyped with the spike protein of the SARS-CoV-2 from those with the G protein of the vesicular stomatitis virus in saline solution and artificial saliva. We report a quantitative analysis of 72 samples of nasopharyngeal exudate in a range of SARS-CoV-2 viral loads, and a descriptive study of another 32 fresh human saliva samples. Sensitivity for classification of exudates was 100% with peak specificity of 87.5% for discernment from PCR-negative but symptomatic cases. Proposed technology is reagent-free, fast, and scalable, and could substantially reduce the number of molecular tests currently required for COVID-19 mass screening strategies even in resource-limited settings. ; The authors would like to gratefully acknowledge the assistance of the members of the Explosive Ordnance Disposal-Chemical, Biological, Radiological & Nuclear (EOD-CBRN) Group of the Spanish National Police, whose identities cannot be disclosed, and who are represented here by JMNG. The authors also thank Manuel Guerrero-Claro for his technical assistance with the implementation of the neural networks, and the companies CAMBRICO BIOTECH (Sevilla, Spain), CER "Dr. Gregorio Medina Blanco" (Espartinas, Sevilla, Spain) and SAMU (Sevilla, Spain) for their collaboration in the collection of human samples. This research was funded by Grants Number COV20-00080 and COV20-00173 of the 2020 Emergency Call for Research Projects about the SARS-CoV-2 virus and the COVID-19 disease of the ...
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: English
تدمد: 2045-2322
العلاقة: info:eu-repo/grantAgreement/ISCIII//COV20-00080; info:eu-repo/grantAgreement/ISCIII//COV20-00173; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094465-J-I00/ES/RECABLEANDO EL CEREBRO EN VISION BIONICA/; http://hdl.handle.net/10498/26562Test
DOI: 10.1038/s41598-022-06393-3
الإتاحة: https://doi.org/10.1038/s41598-022-06393-3Test
http://hdl.handle.net/10498/26562Test
حقوق: Atribución 4.0 Internacional ; http://creativecommons.org/licenses/by/4.0Test/ ; info:eu-repo/semantics/openAccess
رقم الانضمام: edsbas.14881223
قاعدة البيانات: BASE
الوصف
تدمد:20452322
DOI:10.1038/s41598-022-06393-3