دورية أكاديمية
Decreased steroidogenic enzyme activity in benign adrenocortical tumors is more pronounced in bilateral lesions as determined by steroid profiling in LC-MS/MS during ACTH stimulation test
| العنوان: | Decreased steroidogenic enzyme activity in benign adrenocortical tumors is more pronounced in bilateral lesions as determined by steroid profiling in LC-MS/MS during ACTH stimulation test |
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| المؤلفون: | Bonnet-Serrano, Fidéline, Barat, Maxime, Vaczlavik, Anna, Jouinot, Anne, Bouys, Lucas, Laguillier-Morizot, Christelle, Zientek, Corinne, Simonneau, Catherine, Larger, Etienne, Guignat, Laurence, Groussin, Lionel, Assié, Guillaume, Guibourdenche, Jean, Nicolis, Ioannis, Menet, Marie-Claude, Bertherat, Jérôme |
| المساهمون: | Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Physiopathologie et pharmacotoxicologie placentaire humaine : Microbiote pré & post natal (3PHM - UMR-S 1139), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Hôpital Cochin AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Biostatistique, traitement et modélisation des données biologiques = Biostatistic, Biological Data treatment and Modelisation (BioSTM - URP_7537), Université Paris Cité (UPCité), Institut de Chimie Physique (ICP), Institut de Chimie - CNRS Chimie (INC-CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), ANR-18-CE14-0008,STEROMICS,Steroïdogénomique de l'hypersécrétion des stéroïdes surrénaliens(2018) |
| المصدر: | EISSN: 2049-3614 ; Endocrine Connections ; https://hal.science/hal-03831131Test ; Endocrine Connections, 2022, 11 (8), pp.e220063. ⟨10.1530/EC-22-0063⟩ |
| بيانات النشر: | HAL CCSD BioScientifica Ltd. |
| سنة النشر: | 2022 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio], [STAT.AP]Statistics [stat]/Applications [stat.AP] |
| الوصف: | International audience ; Objective: Large response of steroid precursors, including 17-hydroxyprogesterone, to adrenocorticotropic hormone (ACTH) has been described in adrenocortical tumors, suggesting the existence of intra-tumoral enzymatic deficiencies. This study aimed to compare steroidogenesis enzymes activity in unilateral and bilateral benign tumors using serum steroid profiling in liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in the basal state and after ACTH 1-24 stimulation. Design and methods: A serum profile of seven consecutive adrenal steroids was determined in LC-MS/MS in the basal state (T0) and after ACTH 1-24 stimulation (T60) in 35 patients with bilateral adrenocortical tumors (BL), 38 patients with unilateral tumors (UL) and 37 control subjects (CT). Response amplitude of each individual steroid was evaluated by T60/T0 ratio, whereas enzymatic activity was assessed by the downstream/upstream steroid ratio. Adrenal volume was quantified by a semi-automatic segmentation method. Results: For the seven steroids assayed, the amplitude of response to ACTH was higher in BL than in UL and in CT. The difference between BL and UL persisted even after matching patients on adrenal volume. On glucocorticoids pathway, enzymatic activity of CYP11B1 was significantly decreased in BL (78.3 (43.1-199.4)) in comparison to both UL (122.7 (13.8-228.4), P = 0.0002) and CT (186.8 (42.1-1236.3), P \textless 0.0001). On mineralocorticoids and androgens pathways, the enzymatic activity of CYP11B2 and CYP17A1-17,20 lyase was also lower in BL than UL and CT. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03831131; https://hal.science/hal-03831131Test |
| DOI: | 10.1530/EC-22-0063 |
| الإتاحة: | https://doi.org/10.1530/EC-22-0063Test https://hal.science/hal-03831131Test |
| رقم الانضمام: | edsbas.13E701F0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1530/EC-22-0063 |
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