التفاصيل البيبلوغرافية
| العنوان: |
Cell-Instructive Surface Gradients of Photoresponsive Amyloid-like Fibrils |
| المؤلفون: |
Adriana Maria Ender (11425773), Kübra Kaygisiz (8375202), Hans-Joachim Räder (5829056), Franz J. Mayer (11425776), Christopher V. Synatschke (1715782), Tanja Weil (1326315) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Cell Biology, Physiology, Biotechnology, Ecology, Immunology, Developmental Biology, Cancer, Space Science, Chemical Sciences not elsewhere classified, sheet forming peptide, photosensitive nitrobenzyl linker, complex biological systems, binding peptide rgd, bioactive molecules play, instructive surface gradients, rgd epitope via, modulate cell adhesion, like fibrils gradients, bioactive molecules, macroscopic gradients, like fibrils, adhesion properties, model epitope, epitope density, rgd gradient, tissue regeneration, scaffolds functionalized, photoresponsive nanofibrils |
| الوصف: |
Gradients of bioactive molecules play a crucial role in various biological processes like vascularization, tissue regeneration, or cell migration. To study these complex biological systems, it is necessary to control the concentration of bioactive molecules on their substrates. Here, we created a photochemical strategy to generate gradients using amyloid-like fibrils as scaffolds functionalized with a model epitope, that is, the integrin-binding peptide RGD, to modulate cell adhesion. The self-assembling β-sheet forming peptide (CKFKFQF) was connected to the RGD epitope via a photosensitive nitrobenzyl linker and assembled into photoresponsive nanofibrils. The fibrils were spray-coated on glass substrates and macroscopic gradients were generated by UV-light over a centimeter-scale. We confirmed the gradient formation using matrix-assisted laser desorption ionization mass spectroscopy imaging (MALDI-MSI), which directly visualizes the molecular species on the surface. The RGD gradient was used to instruct cells. In consequence, A549 adapted their adhesion properties in dependence of the RGD-epitope density. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Cell-Instructive_Surface_Gradients_of_Photoresponsive_Amyloid-like_Fibrils/16611246Test |
| DOI: |
10.1021/acsbiomaterials.1c00889.s001 |
| الإتاحة: |
https://doi.org/10.1021/acsbiomaterials.1c00889.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.12CEAEEB |
| قاعدة البيانات: |
BASE |
