التفاصيل البيبلوغرافية
| العنوان: |
Cross-protection induced by highly conserved human B, CD4+, and CD8+ T-cell epitopes-based vaccine against severe infection, disease, and death caused by multiple SARS-CoV-2 variants of concern |
| المؤلفون: |
Prakash, Swayam, Dhanushkodi, Nisha R., Zayou, Latifa, Ibraim, Izabela Coimbra, Quadiri, Afshana, Coulon, Pierre Gregoire, Tifrea, Delia F., Suzer, Berfin, Shaik, Amin Mohammed, Chilukuri, Amruth, Edwards, Robert A., Singer, Mahmoud, Vahed, Hawa, Nesburn, Anthony B., Kuppermann, Baruch D., Ulmer, Jeffrey B., Gil, Daniel, Jones, Trevor M., BenMohamed, Lbachir |
| المساهمون: |
National Institute of Allergy and Infectious Diseases |
| المصدر: |
Frontiers in Immunology ; volume 15 ; ISSN 1664-3224 |
| بيانات النشر: |
Frontiers Media SA |
| سنة النشر: |
2024 |
| المجموعة: |
Frontiers (Publisher - via CrossRef) |
| الوصف: |
Background The coronavirus disease 2019 (COVID-19) pandemic has created one of the largest global health crises in almost a century. Although the current rate of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections has decreased significantly, the long-term outlook of COVID-19 remains a serious cause of morbidity and mortality worldwide, with the mortality rate still substantially surpassing even that recorded for influenza viruses. The continued emergence of SARS-CoV-2 variants of concern (VOCs), including multiple heavily mutated Omicron sub-variants, has prolonged the COVID-19 pandemic and underscores the urgent need for a next-generation vaccine that will protect from multiple SARS-CoV-2 VOCs. Methods We designed a multi-epitope-based coronavirus vaccine that incorporated B, CD4 + , and CD8 + T- cell epitopes conserved among all known SARS-CoV-2 VOCs and selectively recognized by CD8 + and CD4 + T-cells from asymptomatic COVID-19 patients irrespective of VOC infection. The safety, immunogenicity, and cross-protective immunity of this pan-variant SARS-CoV-2 vaccine were studied against six VOCs using an innovative triple transgenic h-ACE-2-HLA-A2/DR mouse model. Results The pan-variant SARS-CoV-2 vaccine (i) is safe , (ii) induces high frequencies of lung-resident functional CD8 + and CD4 + T EM and T RM cells , and (iii) provides robust protection against morbidity and virus replication. COVID-19-related lung pathology and death were caused by six SARS-CoV-2 VOCs: Alpha (B.1.1.7), Beta (B.1.351), Gamma or P1 (B.1.1.28.1), Delta (lineage B.1.617.2), and Omicron (B.1.1.529). Conclusion A multi-epitope pan-variant SARS-CoV-2 vaccine bearing conserved human B- and T- cell epitopes from structural and non-structural SARS-CoV-2 antigens induced cross-protective immunity that facilitated virus clearance, and reduced morbidity, COVID-19-related lung pathology, and death caused by multiple SARS-CoV-2 VOCs. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| DOI: |
10.3389/fimmu.2024.1328905 |
| DOI: |
10.3389/fimmu.2024.1328905/full |
| الإتاحة: |
https://doi.org/10.3389/fimmu.2024.1328905Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.11A5626C |
| قاعدة البيانات: |
BASE |
