دورية أكاديمية
DNA demethylation agent 5azadC downregulates HPV16 E6 expression in cervical cancer cell lines independently of TBX2 expression
| العنوان: | DNA demethylation agent 5azadC downregulates HPV16 E6 expression in cervical cancer cell lines independently of TBX2 expression |
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| المؤلفون: | Perrard, Jérôme, Morel, Adrien, Meznad, Koceila, Paget‑bailly, Philippe, Dalstein, Véronique, Guenat, David, Mourareau, Celine, Clavel, Christine, E., Fauconnet, Sylvie, Baguet, Aurélie, Mougin, Christiane, Pretet, Jean‑luc |
| المساهمون: | Laboratoire d'Excellence : Lipoprotéines et Santé : prévention et Traitement des maladies Inflammatoires et du Cancer (LabEx LipSTIC), École Pratique des Hautes Études (EPHE), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut Gustave Roussy (IGR)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer Georges-François Leclerc Dijon (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-Université de Montpellier (UM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang Bourgogne-Franche-Comté (EFS BFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté COMUE (UBFC)-Université Bourgogne Franche-Comté COMUE (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centro de Investigación en Genética y Genómica-CIGGUR, Grupo GENIUROS, Universidad del Rosario, Bogota, Pathologies Pulmonaires et Plasticité Cellulaire - UMR-S 1250 (P3CELL), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital universitaire Robert Debré Reims (CHU Reims), Centre National de Référence des Papillomavirus (CNRP), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon) |
| المصدر: | ISSN: 1792-1074. |
| بيانات النشر: | HAL CCSD Spandidos Publications |
| سنة النشر: | 2019 |
| المجموعة: | Université de Reims Champagne-Ardenne: Archives Ouvertes (HAL) |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; HPV16 is the most carcinogenic human papilloma-virus and causes >50% of cervical cancers, the majority of anal cancers and 30% of oropharyngeal squamous cell carcinomas. HPV carcinogenesis relies on the continuous expression of the two main viral oncoproteins E6 and E7 that target >150 cellular proteins. Among them, epigenetic modifiers, including DNA Methyl Transferases (DNMT), are dysregulated, promoting an aberrant methylation pattern in HPV-positive cancer cells. It has been previously reported that the treatment of HPV-positive cervical cancer cells with DNMT inhibitor 5-aza-2'-deoxycytidine (5azadC) caused the downregulation of E6 expression due to mRNA destabilization that was mediated by miR-375. Recently, the T-box transcription factor 2 (TBX2) has been demonstrated to repress HPV LCR activity. In the current study, the role of TBX2 in E6 repression was investigated in HPV16 cervical cancer cell lines following 5azadC treatment. A decrease of E6 expression was accompanied by p53 and p21 restoration. While TBX2 mRNA was upregulated in 5azadC-treated SiHa and Ca Ski cells, TBX2 protein was not detectable. Furthermore, the overexpression of TBX2 protein in cervical cancer cells did not allow the repression of E6 expression. The TBX2 transcription factor is therefore unlikely to be associated with the repression of E6 following 5azadC treatment of SiHa and Ca Ski cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-02451680; https://hal.univ-reims.fr/hal-02451680Test; https://hal.univ-reims.fr/hal-02451680/documentTest; https://hal.univ-reims.fr/hal-02451680/file/PerrardJerome_OncoLetters_2019.pdfTest |
| DOI: | 10.3892/ol.2019.11158 |
| الإتاحة: | https://doi.org/10.3892/ol.2019.11158Test https://hal.univ-reims.fr/hal-02451680Test https://hal.univ-reims.fr/hal-02451680/documentTest https://hal.univ-reims.fr/hal-02451680/file/PerrardJerome_OncoLetters_2019.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.114DBF14 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3892/ol.2019.11158 |
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