Deterministic progenitor behavior and unitary production of neurons in the neocortex
| العنوان: | Deterministic progenitor behavior and unitary production of neurons in the neocortex |
|---|---|
| المؤلفون: | Gao, Peng, Postiglione, Maria Pia, Krieger, Teresa G, Hernandez, Luisirene, Wang, Chao, Han, Zhi, Streicher, Carmen, Papusheva, Ekaterina, Insolera, Ryan, Chugh, Kritika, Kodish, Oren, Huang, Kun, Simons, Benjamin D, Luo, Liqun, Hippenmeyer, Simon, Shi, Song-Hai |
| المساهمون: | Simons, Benjamin [0000-0002-3875-7071], Apollo - University of Cambridge Repository |
| المصدر: | Cell |
| بيانات النشر: | Elsevier BV, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Neurons, Mice, Otx Transcription Factors, nervous system, Staining and Labeling, Biochemistry, Genetics and Molecular Biology(all), Neurogenesis, Stem Cells, 570 Life sciences, biology, Animals, Neocortex, Neuroglia, Article |
| الوصف: | Summary Radial glial progenitors (RGPs) are responsible for producing nearly all neocortical neurons. To gain insight into the patterns of RGP division and neuron production, we quantitatively analyzed excitatory neuron genesis in the mouse neocortex using Mosaic Analysis with Double Markers, which provides single-cell resolution of progenitor division patterns and potential in vivo. We found that RGPs progress through a coherent program in which their proliferative potential diminishes in a predictable manner. Upon entry into the neurogenic phase, individual RGPs produce ∼8–9 neurons distributed in both deep and superficial layers, indicating a unitary output in neuronal production. Removal of OTX1, a transcription factor transiently expressed in RGPs, results in both deep- and superficial-layer neuron loss and a reduction in neuronal unit size. Moreover, ∼1/6 of neurogenic RGPs proceed to produce glia. These results suggest that progenitor behavior and histogenesis in the mammalian neocortex conform to a remarkably orderly and deterministic program. Graphical Abstract Highlights • Radial glial progenitors (RGPs) progress through a coherent proliferation program • Individual RGPs produce a unitary output of neurons during the neurogenic phase • OTX1 deletion in RGPs leads to a reduction in neuronal unit size • A defined fraction of about one in six RGPs proceeds to gliogenesis after neurogenesis Individual radial glial progenitors divide a defined number of times to produce excitatory neurons that initially enter the deep layers of the neocortex before populating the more superficial layers. A constant fraction of these neurons go on to produce glia, thereby maintaining the balance between the two cell types. |
| وصف الملف: | application/pdf; text |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::fc381c6675c0091f40a507db90c66657Test https://www.repository.cam.ac.uk/handle/1810/246217Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....fc381c6675c0091f40a507db90c66657 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |