Characterization of human and mouse c-fes cDNA clones and identification of the 5' end of the gene
| العنوان: | Characterization of human and mouse c-fes cDNA clones and identification of the 5' end of the gene |
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| المؤلفون: | Myriam Alcalay, Antolini F, Wj, Ven, Lanfrancone L, Grignani F, Pg, Pelicci |
| المصدر: | Scopus-Elsevier Europe PubMed Central |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Base Sequence, Placenta, Molecular Sequence Data, Restriction Mapping, DNA, Exons, Protein-Tyrosine Kinases, Methylation, Cell Line, Mice, Proto-Oncogene Proteins c-fes, Pregnancy, Proto-Oncogene Proteins, Sequence Homology, Nucleic Acid, Proto-Oncogenes, Animals, Humans, Female, Amino Acid Sequence, RNA, Messenger, Gene Library |
| الوصف: | cDNA clones of the human c-fes mRNA were isolated. Nucleotide analysis showed that c-fes mRNA contains a 2514 nucleotide open reading frame, which could encode for a 93 kDa protein, and both 5' and 3' v-fes nonhomologous sequences. Primer extension experiments confirmed that the longest isolated cDNAs are about the same length as the entire human c-fes mRNA. Sequence comparison between human c-fes cDNA and the corresponding genomic regions identified a 5' viral-non homologous exon (exon 1) located 491 bp upstream from the first v-fes homologous exon. The genomic region surrounding c-fes exon 1 contains a CpG island and acts as a promoter in vitro. Analysis of the 5' end of mouse c-fes cDNA suggested that the 5' human and mouse gene structure are similar. |
| تدمد: | 0950-9232 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::e7c2344a0963c0dcd5fe04bc3ca28dd4Test https://pubmed.ncbi.nlm.nih.gov/2179816Test |
| رقم الانضمام: | edsair.pmid.dedup....e7c2344a0963c0dcd5fe04bc3ca28dd4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09509232 |
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