Extracellular Acidosis Modulates the Expression of Epithelial-Mesenchymal Transition (EMT) Markers and Adhesion of Epithelial and Tumor Cells
| العنوان: | Extracellular Acidosis Modulates the Expression of Epithelial-Mesenchymal Transition (EMT) Markers and Adhesion of Epithelial and Tumor Cells |
|---|---|
| المؤلفون: | Riemann, Anne, Rauschner, Mandy, Gießelmann, Marina, Reime, Sarah, Haupt, Verena, Thews, Oliver |
| المصدر: | Neoplasia: An International Journal for Oncology Research, Vol 21, Iss 5, Pp 450-458 (2019) Neoplasia (New York, N.Y.) |
| بيانات النشر: | Elsevier, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, Original article, Scd2, stearoyl-CoA desaturase 2, Epithelial-Mesenchymal Transition, Snai1, snail family transcriptional repressor 1, Twist1, twist family bHLH transcription factor 1, lcsh:RC254-282, Cell Adhesion, Animals, Humans, Vimentin, Fh, fumarate hydratase, Acsl1, acyl-CoA synthetase long-chain family member 1, Zeb2, zinc finger E-box binding homeobox 2, Zeb1, zinc finger E-box binding homeobox 1, Cadherins, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Gpi, glucose-6-phosphate isomerase, Rats, NGS, next-generation sequencing, Gene Expression Regulation, Neoplastic, Idh2, isocitrate dehydrogenase 2, Scd1, stearoyl-CoA desaturase 1, MIBG, meta-iodobenzylguanidine, Acidosis, Biomarkers |
| الوصف: | Epithelial-to-mesenchymal transition (EMT) is an important process of tumor progression associated with increased metastatic potential. EMT can be activated by external triggers such as cytokines or metabolic parameters (e.g. hypoxia). Since extracellular acidosis is a common finding in tumors, the aim of the study is to analyze its impact on the expression of EMT markers in vitro and in vivo as well as the functional impact on cell adhesion. Therefore, three tumor and two normal epithelial cell lines were incubated for 24 h at pH 6.6 and the expression of EMT markers was studied. In addition, mRNA expression of transcription and metabolic factors related to EMT was measured as well as the functional impact on cell adhesion, either during acidic incubation or after priming cells in an acidic milieu. E-cadherin and N-cadherin were down-regulated in all tumor and normal cell lines studied, whereas vimentin expression increased in only two tumor and one normal cell line. Down-regulation of the cadherins was seen in total protein and to a lesser extent in surface protein. In vivo an increase in N-cadherin and vimentin expression was found. Acidosis up-regulated Twist1 and Acsl1 but down-regulated fumarate hydratase (Fh). Cell adhesion during acidic incubation decreased in AT1 prostate carcinoma cells whereas preceding acidic priming increased their subsequent adhesion. Low tumor pH is able to modulate the expression EMT-related proteins and by this may affect the stability of the tissue structure. |
| اللغة: | English |
| تدمد: | 1476-5586 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::d204e64efdc5d7dd32703734bfd194d2Test http://www.sciencedirect.com/science/article/pii/S1476558618306705Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....d204e64efdc5d7dd32703734bfd194d2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765586 |
|---|