Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes
| العنوان: | Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes |
|---|---|
| المؤلفون: | Bhandage, Amol K., Jin, Zhe, Korol, Sergiy V., Tafreshiha, Atieh S., Gohel, Priya, Hellgren, Charlotte, Espes, Daniel, Carlsson, Per-Ola, Sundström-Poromaa, Inger, Birnir, Bryndis |
| المصدر: | PLoS ONE, Vol 13, Iss 12, p e0208981 (2018) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Voltage-Gated Ion Channels, Male, Physiology, Maternal Health, Cell Membranes, Gene Expression, Biochemistry, Ion Channels, Endocrinology, Pregnancy, Animal Cells, Medicine and Health Sciences, Child, Voltage-Gated Calcium Channels, Physics, Biochemistry and Molecular Biology, Obstetrics and Gynecology, Middle Aged, Calcium Release Activated Calcium Channels, Electrophysiology, Child, Preschool, Endokrinologi och diabetes, Physical Sciences, Type 1 Diabetes, Medicine, Female, Cellular Structures and Organelles, Cellular Types, Research Article, Adult, Adolescent, Endocrine Disorders, Immune Cells, Science, Immunology, Biophysics, Neurophysiology, Endocrinology and Diabetes, Young Adult, Diabetes Mellitus, Genetics, Humans, RNA, Messenger, Biology and Life Sciences, Membrane Proteins, Proteins, Infant, Cell Biology, Diabetes Mellitus, Type 1, Gene Expression Regulation, Metabolic Disorders, Case-Control Studies, Leukocytes, Mononuclear, Women's Health, Calcium, Calcium Channels, Biokemi och molekylärbiologi, Neuroscience |
| الوصف: | Calcium (Ca2+) is an important ion in physiology and is found both outside and inside cells. The intracellular concentration of Ca2+ is tightly regulated as it is an intracellular signal molecule and can affect a variety of cellular processes. In immune cells Ca2+ has been shown to regulate e.g. gene transcription, cytokine secretion, proliferation and migration. Ca2+ can enter the cytoplasm either from intracellular stores or from outside the cells when Ca2+ permeable ion channels in the plasma membrane open. The Ca2+ release-activated (CRAC) channel is the most prominent Ca2+ ion channel in the plasma membrane. It is formed by ORAI1-3 and the channel is opened by the endoplasmic reticulum Ca2+ sensor proteins stromal interaction molecules (STIM) 1 and 2. Another group of Ca-2(+) channels in the plasma membrane are the voltage-gated Ca2+ (Ca-V) channels. We examined if a change in immunological tolerance is accompanied by altered ORAI, STIM and Ca-V gene expression in peripheral blood mononuclear cells (PBMCs) in pregnant women and in type 1 diabetic individuals. Our results show that in pregnancy and type 1 diabetes ORAI1-3 are up-regulated whereas STIM1 and 2 are down-regulated in pregnancy but only STIM2 in type 1 diabetes. Expression of L-, P/Q-, R- and T-type voltage-gated Ca2+ channels was detected in the PBMCs where the Ca(V)2.3 gene was up-regulated in pregnancy and type 1 diabetes whereas the Ca(V)2.1 and Ca(V)3.2 genes were up-regulated only in pregnancy and the Ca(V)1.3 gene in type 1 diabetes. The results are consistent with that expression of ORAI, STIM and Ca-V genes correlate with a shift in immunological status of the individual in health, as during pregnancy, and in the autoimmune disease type 1 diabetes. Whether the changes are in general protective or in type 1 diabetes include some pathogenic components remains to be clarified. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::ab3c5d7e2f621a68308ecedabdb0c6b0Test https://doaj.org/article/fcf1445579af46809b0dcbb0ceaeb668Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....ab3c5d7e2f621a68308ecedabdb0c6b0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|