UVR8-mediated inhibition of shade avoidance involves HFR1 stabilization in Arabidopsis
| العنوان: | UVR8-mediated inhibition of shade avoidance involves HFR1 stabilization in Arabidopsis |
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| المؤلفون: | Tavridou, Eleni, Schmid-Siegert, Emanuel, Fankhauser, Christian, Ulm, Roman |
| المصدر: | PLoS Genetics, Vol 16, Iss 5, p e1008797 (2020) PLoS genetics, vol. 16, no. 5, pp. e1008797 PLoS Genetics PLOS Genetics, Vol. 16, No 5 (2020) P. e1008797 |
| بيانات النشر: | Public Library of Science (PLoS), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Fruit and Seed Anatomy, Light, Chromosomal Proteins, Non-Histone, Arabidopsis, Gene Expression, Plant Science, QH426-470, ddc:590, Gene Expression Regulation, Plant, Basic Helix-Loop-Helix Transcription Factors, Plant Growth and Development, Protein Stability, Physics, Transcriptional Control, Plant Anatomy, Eukaryota, Plants, Hypocotyl, Chromatin, Physical sciences, DNA-Binding Proteins, ddc:580, Embryogenesis, Research Article, Ultraviolet radiation, Visible Light, Ultraviolet Rays, Ubiquitin-Protein Ligases, Plant Development, Down-Regulation, Research and Analysis Methods, Electromagnetic radiation, Gene Types, Genetics, White Light, Gene Regulation, Molecular Biology Techniques, Molecular Biology, Plant Embryo Anatomy, Arabidopsis Proteins, Organisms, Biology and Life Sciences, Marker Genes, Arabidopsis/drug effects, Arabidopsis/genetics, Arabidopsis/metabolism, Arabidopsis Proteins/chemistry, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Basic Helix-Loop-Helix Transcription Factors/chemistry, Basic Helix-Loop-Helix Transcription Factors/genetics, Basic Helix-Loop-Helix Transcription Factors/metabolism, Chromatin/metabolism, Chromosomal Proteins, Non-Histone/genetics, Chromosomal Proteins, Non-Histone/metabolism, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Gene Expression Regulation, Plant/radiation effects, Proteolysis, Transcription Factors/genetics, Transcription Factors/metabolism, Ubiquitin-Protein Ligases/genetics, Ubiquitin-Protein Ligases/metabolism, Ultraviolet Rays/adverse effects, Seedlings, Regulator Genes, Ultraviolet B, Plant Embryogenesis, Developmental Biology, Transcription Factors |
| الوصف: | Sun-loving plants perceive the proximity of potential light-competing neighboring plants as a reduction in the red:far-red ratio (R:FR), which elicits a suite of responses called the “shade avoidance syndrome” (SAS). Changes in R:FR are primarily perceived by phytochrome B (phyB), whereas UV-B perceived by UV RESISTANCE LOCUS 8 (UVR8) elicits opposing responses to provide a counterbalance to SAS, including reduced shade-induced hypocotyl and petiole elongation. Here we show at the genome-wide level that UVR8 broadly suppresses shade-induced gene expression. A subset of this gene regulation is dependent on the UVR8-stabilized atypical bHLH transcription regulator LONG HYPOCOTYL IN FAR-RED 1 (HFR1), which functions in part redundantly with PHYTOCHROME INTERACTING FACTOR 3-LIKE 1 (PIL1). In parallel, UVR8 signaling decreases protein levels of the key positive regulators of SAS, namely the bHLH transcription factors PHYTOCHROME INTERACTING FACTOR 4 (PIF4) and PIF5, in a COP1-dependent but HFR1-independent manner. We propose that UV-B antagonizes SAS via two mechanisms: degradation of PIF4 and PIF5, and HFR1- and PIL1-mediated inhibition of PIF4 and PIF5 function. This work highlights the importance of typical and atypical bHLH transcription regulators for the integration of light signals from different photoreceptors and provides further mechanistic insight into the crosstalk of UVR8 signaling and SAS. Author summary Sunlight provides the energy that powers photosynthesis and is thus of the utmost importance for plant growth and development. Plants grow in constantly changing environments, including highly variable light conditions. They have evolved specific light sensors (photoreceptors) that allow optimization of photosynthesis and survival, and adjusting growth and development to the prevailing environment. An intriguing example is the perception of competitors due to changes in the light transmitted and reflected by their leaves. As red light, but not far-red, is strongly absorbed by photosynthetic pigments, the red:far-red ratio (R:FR) is strongly reduced in the presence of competitors which is sensed by the phytochrome red/far-red photoreceptors. Detection of competitors results in stem elongation in shade-intolerant plants to overtop the neighbors and reach full sunlight (shade avoidance syndrome). UV-B sensed by the UVR8 photoreceptor inhibits shade avoidance by reducing the abundance and activity of specific transcriptional regulators PIF4 and PIF5. We have discovered an important role for an atypical transcriptional regulator HFR1 that is stabilized under UV-B, strongly contributing to the UVR8 photoreceptor-mediated repression of shade responses by counteracting PIF4 and PIF5 activities. This contributes significantly to a deeper mechanistic understanding of how plants integrate information from a complex light environment. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1553-7404 1553-7390 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::0545b92567db39a9ed60551075590f7fTest https://doaj.org/article/0e9d7e2ea96b4a92a1377c80f65a3f8fTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....0545b92567db39a9ed60551075590f7f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15537404 15537390 |
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