Harnessing a P450 fatty acid decarboxylase from
| العنوان: | Harnessing a P450 fatty acid decarboxylase from |
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| المؤلفون: | Jong-Won, Lee, Narayan P, Niraula, Cong T, Trinh |
| المصدر: | Metabolic Engineering Communications |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Terminal alkene, Alanine scan, Escherichia coli, Macrococcus caseolyticus, P450 decarboxylase, Article, Protein homology modeling |
| الوصف: | Alkenes are industrially important platform chemicals with broad applications. In this study, we report a direct microbial biosynthesis of terminal alkenes from fermentable sugars by harnessing a P450 fatty acid (FA) decarboxylase from Macrococcus caseolyticus (OleTMC). We first characterized OleTMC and demonstrated its in vitro H2O2-independent activities towards linear C10:0-C18:0 FAs, with higher activity for C16:0-C18:0 FAs. Next, we engineered a de novo alkene biosynthesis pathway, consisting of OleTMC and an engineered E. coli thioesterase (TesA) with compatible substrate specificities, and introduced this pathway into E. coli for terminal alkene biosynthesis from glucose. The recombinant E. coli EcNN101 produced a total of 17.78 ± 0.63 mg/L odd-chain terminal alkenes, comprising of 0.9% ± 0.5% C11 alkene, 12.7% ± 2.2% C13 alkene, 82.7% ± 1.7% C15 alkene, and 3.7% ± 0.8% C17 alkene, and a yield of 0.87 ± 0.03 (mg/g) on glucose. To improve alkene production, we identified and overcame the electron transfer limitation in OleTMC, by introducing a two-component redox system, consisting of a putidaredoxin reductase (CamA) and a putidaredoxin (CamB) from Pseudomonas putida, into EcNN101, and demonstrated the alkene production increased ~2.8 fold. Finally, to better understand the substrate specificities of OleTMC observed, we employed in silico protein modeling to illuminate the functional role of FA binding pocket. Highlights • Characterize a novel P450 fatty acid decarboxylase, OleTMC, for alkene biosynthesis. • Demonstrate alkene biosynthesis directly from glucose in a recombinant E. coli. • Alleviate electron transfer limitation in OleTMC for improved alkene production. • Use in silico protein modeling to illuminate substrate specificity of OleTMC. |
| تدمد: | 2214-0301 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid________::e95c2c7e207413ec97d38573838c9baaTest https://pubmed.ncbi.nlm.nih.gov/30197865Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid..........e95c2c7e207413ec97d38573838c9baa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22140301 |
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