Skin models for cutaneous melioidosis reveal

التفاصيل البيبلوغرافية
العنوان: Skin models for cutaneous melioidosis reveal
المؤلفون: Joanne Wei Kay, Ku, Supatra Tharinee, Marsh, Mui Hoon, Nai, Kim Samirah, Robinson, Daniel Eng Thiam, Teo, Franklin Lei, Zhong, Katherine A, Brown, Thiam Chye, Lim, Chwee Teck, Lim, Yunn-Hwen, Gan
المصدر: Emerging Microbes & Infections
article-version (VoR) Version of Record
سنة النشر: 2021
مصطلحات موضوعية: Keratinocytes, skin, Burkholderia pseudomallei, integumentary system, Burkholderia, Inflammasomes, keratinocyte, Models, Biological, Melioidosis, inflammasome, Humans, Wounds and Injuries, Epidermis, Cells, Cultured, Research Article
الوصف: Melioidosis is a serious infectious disease endemic in Southeast Asia, Northern Australia and has been increasingly reported in other tropical and subtropical regions in the world. Percutaneous inoculation through cuts and wounds on the skin is one of the major modes of natural transmission. Despite cuts in skin being a major route of entry, very little is known about how the causative bacterium Burkholderia pseudomallei initiates an infection at the skin and the disease manifestation at the skin known as cutaneous melioidosis. One key issue is the lack of suitable and relevant infection models. Employing an in vitro 2D keratinocyte cell culture, a 3D skin equivalent fibroblast-keratinocyte co-culture and ex vivo organ culture from human skin, we developed infection models utilizing surrogate model organism Burkholderia thailandensis to investigate Burkholderia-skin interactions. Collectively, these models show that the bacterial infection was largely limited at the wound’s edge. Infection impedes wound closure, triggers inflammasome activation and cellular extrusion in the keratinocytes as a potential way to control bacterial infectious load at the skin. However, extensive infection over time could result in the epidermal layer being sloughed off, potentially contributing to formation of skin lesions.
تدمد: 2222-1751
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::18b676f0f3004dcf9b215e5a89f60bc9Test
https://pubmed.ncbi.nlm.nih.gov/34821529Test
حقوق: OPEN
رقم الانضمام: edsair.pmid..........18b676f0f3004dcf9b215e5a89f60bc9
قاعدة البيانات: OpenAIRE