التفاصيل البيبلوغرافية
| العنوان: |
Cumulus cells are potential candidates for cell therapy |
| المؤلفون: |
Mohammed, E.E., Yilmaz, S., Akcin, O.A., Nalbantoglu, B., Ficicioglu, C., Şahin, Fikrettin, Coban, E.A. |
| المساهمون: |
Mohammed, E.E., Yilmaz, S., Akcin, O.A., Nalbantoglu, B., Ficicioglu, C., Şahin, Fikrettin, Coban, E.A., Yeditepe Üniversitesi |
| بيانات النشر: |
International Institute of Anticancer Research, 2019. |
| سنة النشر: |
2019 |
| مصطلحات موضوعية: |
Granulosa cells, Oocyte, fungi, Pancreatic cancer, BxPC-3 |
| الوصف: |
Background/Aim: Cumulus cells (CCs) originate from the membrane granulosa cells and surround oocytes during follicle maturation. CCs produce high levels of hyaluronan that targets CD44, which is a major tumorigenic marker. This study aimed to investigate whether CCs have a role in cell therapy by targeting CD44 in pancreatic cancer cells. Materials and Methods: CCs were isolated from the oocytes and incubated in a hypoxic environment. BxPC-3 pancreatic cancer cells were treated with CC conditioned media for three days. Results: Conditioned media of CC cells incubated in hypoxic conditions caused a 25% reduction in the viability of BxPC-3 cells. Expression of anti-apoptotic genes was down-regulated, while that of pro-apoptotic genes was upregulated. An increased number of BxPC-3 cells exhibited increased levels of reactive oxygen species and arrested in the synthesis (S) phase of the cell cycle. Conclusion: CCs conditioned medium induced apoptosis of pancreatic cancer cells. © 2019 International Institute of Anticancer Research. All rights reserved. The Authors thank Burcin Asutay for performing flow cytometry and live cell imaging analyses. This work was generously supported by Yeditepe University. |
| اللغة: |
English |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=od_____10007::32333380a7fc36dd11ccbff7712f4fddTest
https://hdl.handle.net/20.500.11831/6522Test |
| حقوق: |
OPEN |
| رقم الانضمام: |
edsair.od.....10007..32333380a7fc36dd11ccbff7712f4fdd |
| قاعدة البيانات: |
OpenAIRE |
