A eutherian-specific microRNA controls the translation of Satb2 in a model of cortical differentiation
| العنوان: | A eutherian-specific microRNA controls the translation of Satb2 in a model of cortical differentiation |
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| المؤلفون: | Silvia Giulia Galfrè, Manuela Helmer-Citterich, Milena Rizzo, Robert Vignali, Laura Poliseno, Irene Appolloni, Keagan Dunville, Francesco Morandin, Paolo Malatesta, Manuella Martins, Andrea Marranci, Marco Pietrosanto, Luca Pandolfini, Marco Terrigno, Federico Cremisi, Alberto Mercatanti |
| المساهمون: | Martins, Manuella, Galfrè, Silvia, Terrigno, Marco, Pandolfini, Luca, Appolloni, Irene, Dunville, Keagan, Marranci, Andrea, Rizzo, Milena, Mercatanti, Alberto, Poliseno, Laura, Morandin, Francesco, Pietrosanto, Marco, Helmer-Citterich, Manuela, Malatesta, Paolo, Vignali, Robert, Cremisi, Federico |
| المصدر: | Stem Cell Reports |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Transcription Factor, Biochemistry, corpus callosum, neural stem cell, Mice, Settore BIO/06 - Anatomia Comparata e Citologia, 0302 clinical medicine, RNA interference, developmental timing, 3' Untranslated Regions, neural stem cells, Cerebral Cortex, cell fate, biology, microRNA, Settore BIO/11, Eutheria, Matrix Attachment Region Binding Protein, Gene Expression Regulation, Developmental, Translation (biology), Cell Differentiation, Neural stem cell, Cell biology, mammalian evolution, Corticogenesis, cortex, Human, in vitro corticogenesi, Neurogenesis, 3' Untranslated Region, Cell fate determination, post-transcriptional control, Article, Cell Line, 03 medical and health sciences, SATB2, cell identity, cortical layering, in vitro corticogenesis, miR-catch, Genetics, Animals, Humans, Progenitor cell, Post-transcriptional regulation, Animal, Tumor Suppressor Proteins, Matrix Attachment Region Binding Proteins, Cell Biology, Repressor Protein, Repressor Proteins, MicroRNAs, 030104 developmental biology, Cell fate, biology.protein, Neurogenesi, in vitro corticogenesis, TBR1, 030217 neurology & neurosurgery, Transcription Factors, Developmental Biology |
| الوصف: | Summary Cerebral cortical development is controlled by key transcription factors that specify the neuronal identities in the different layers. The mechanisms controlling their expression in distinct cells are only partially known. We investigated the expression and stability of Tbr1, Bcl11b, Fezf2, Satb2, and Cux1 mRNAs in single developing mouse cortical cells. We observe that Satb2 mRNA appears much earlier than its protein and in a set of cells broader than expected, suggesting an initial inhibition of its translation, subsequently released during development. Mechanistically, Satb2 3′UTR modulates protein translation of GFP reporters during mouse corticogenesis. We select miR-541, a eutherian-specific miRNA, and miR-92a/b as the best candidates responsible for SATB2 inhibition, being strongly expressed in early and reduced in late progenitor cells. Their inactivation triggers robust and premature SATB2 translation in both mouse and human cortical cells. Our findings indicate RNA interference as a major mechanism in timing cortical cell identities. Graphical abstract Highlights • mRNAs for key transcription factors are differentially stable during corticogenesis • miRNAs show a dynamic profile of expression in a model of mouse corticogenesis • miR-541 and miR-92a/b bind Satb2 3′UTR and prevent SATB2 translation • Antagonizing mir-541 and miR-92a/b anticipates SATB2 protein production In this article, Cremisi and colleagues show that post-transcriptional mechanisms are involved in controlling key functional aspects of SATB2-expressing cortical neurons. They show that mir-541, a eutherian-specific miRNA, delays SATB2 protein production in an in vitro model of cortical cell differentiation. These results may explain the heterochronic shift of SATB2 appearance in the eutherian compared with the metatherian cortex. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff37e0b84ee1a85682af8cc2e372669fTest https://hdl.handle.net/11384/94206Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ff37e0b84ee1a85682af8cc2e372669f |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |