MicroRNA-34a Alleviates Gemcitabine Resistance in Pancreatic Cancer by Repression of Cancer Stem Cell Renewal
| العنوان: | MicroRNA-34a Alleviates Gemcitabine Resistance in Pancreatic Cancer by Repression of Cancer Stem Cell Renewal |
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| المؤلفون: | Ge Gao, Jianzhou Liu, Ning Li, Yue Pan, Xufeng Tao, Qing Chen, Kun Li, Junchao Guo, Gary Guishan Xiao, Yongxin Zhao, Vay Liang W. Go |
| المصدر: | Pancreas. 50:1260-1266 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Antimetabolites, Antineoplastic, Epithelial-Mesenchymal Transition, Cell Survival, Endocrinology, Diabetes and Metabolism, Cell, Mice, Nude, Deoxycytidine, Endocrinology, Cell Movement, Cancer stem cell, Cell Line, Tumor, Pancreatic cancer, Internal Medicine, medicine, Animals, Humans, Viability assay, Cell Self Renewal, Receptor, Notch1, Notch 1, Hepatology, Chemistry, medicine.disease, Xenograft Model Antitumor Assays, Gemcitabine, Gene Expression Regulation, Neoplastic, Pancreatic Neoplasms, MicroRNAs, medicine.anatomical_structure, Drug Resistance, Neoplasm, MicroRNA 34a, Cancer research, Stem cell, Carcinoma, Pancreatic Ductal, Signal Transduction, medicine.drug |
| الوصف: | OBJECTIVES This study aimed to enhance the sensitivity of pancreatic ductal adenocarcinoma cells by microRNA-34a (miR-34a)-mediated targeting of Notch 1. METHODS Cell viability was determined by using an MTT (3-(4,5)-dimethylthiahiazo(-2)-3,5-diphenytetrazoliumromide) assay. The expression levels of miR-34a and relevant mRNAs were determined using quantitative polymerase chain reaction. Protein levels were measured by Western blotting. Cellular stemness was assessed by cell invasiveness and sphere formation assays. A transplanted tumor model was established for in vivo experiments. RESULTS MicroRNA-34a enhanced gemcitabine sensitivity both in vivo and in vitro. MicroRNA-34a suppressed the stemness and proliferation of pancreatic cancer stem cells. MicroRNA-34a directly associated with Notch 1, which lies upstream of epithelial-mesenchymal transition signaling pathways. CONCLUSIONS MicroRNA-34a sensitized pancreatic cancer cells to gemcitabine treatment by inhibiting Notch 1 signaling in pancreatic cancer stem cells, indicating that miR-34a has the potential to be developed as a novel therapeutic agent for the treatment of gemcitabine-resistant pancreatic ductal adenocarcinoma cells. |
| تدمد: | 1536-4828 0885-3177 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc44b31821a21c258b90fdd84d36e314Test https://doi.org/10.1097/mpa.0000000000001920Test |
| رقم الانضمام: | edsair.doi.dedup.....fc44b31821a21c258b90fdd84d36e314 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15364828 08853177 |
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