Anti-EFG1 2′-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella
| العنوان: | Anti-EFG1 2′-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella |
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| المؤلفون: | Mariana Henriques, Dalila Mil-Homens, Daniela Araújo, Sónia Silva |
| المساهمون: | Universidade do Minho |
| المصدر: | Molecular Therapy: Nucleic Acids, Vol 27, Iss, Pp 517-523 (2022) Molecular Therapy. Nucleic Acids Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | 2′-OMethyl chemical modification, 0303 health sciences, Ciências Naturais::Ciências Biológicas, Science & Technology, 030306 microbiology, fungi, Ciências Biológicas [Ciências Naturais], RM1-950, candidiasis, 3. Good health, virulence, 03 medical and health sciences, Galleria mellonella, Drug Discovery, otorhinolaryngologic diseases, Molecular Medicine, Original Article, Therapeutics. Pharmacology, antisense oligonucleotides, 030304 developmental biology |
| الوصف: | EFG1 is a central transcriptional regulator of filamentation that is an important virulence factor of Candida albicans. This study serves to assess in vivo the applicability of the anti-EFG1 2-OMethylRNA oligomer for inhibiting Candida albicans filamentation and to attenuate candidiasis, using the Galleria mellonella model. For that, larvae infected with a lethal concentration of C. albicans cells were treated with a single-dose and with a double-dose of the anti-EFG1 2OMe oligomer (at 40 and 100 nM). The anti-EFG1 2OMe oligomer toxicity and effect on larvae survival was evaluated. No evidence of anti-EFG1 2OMe oligomer toxicity was observed and the treatment with double-dose of 2OMe oligomer empowered the larvae survival over 24 h (by 90-100%) and prolonged its efficacy until 72 h of infection (by 30%). Undoubtedly, this work validates the in vivo therapeutic potential of anti-EFG1 2OMe oligomer for controlling C. albicans infections. This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation ( NORTE-01-0145-FEDER-000004 ) funded by the European Regional Development Fund under the scope of Norte2–20 – Programa Operacional Regional do Norte and Daniela Eira Araújo [ SFRH/BD/121417/2016 ] PhD grant. The authors also acknowledge the project funding by the “ 02/SAICT/2017 – Projetos de Investigação Científica e Desenvolvimento Tecnológico (IC&DT) – POCI-01-0145-FEDER-028893.” Funding received by IBB – Institute for Bioengineering and Biosciences from FCT ( UID/BIO/04565/2020 ) and Programa Operacional Regional de Lisboa 2020 (project no. 007317 ) is also acknowledged. We acknowledge Dr Lucília Goreti Pinto, Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho , for processing and sectioning tissue samples of G. mellonella. info:eu-repo/semantics/publishedVersion |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2162-2531 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb8ec732ce2e08545126fdcdd6cd483eTest http://www.sciencedirect.com/science/article/pii/S2162253121003218Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fb8ec732ce2e08545126fdcdd6cd483e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21622531 |
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