Identification, cDNA Cloning, and Characterization of Luteinizing Hormone Beta Subunit (lhb) Gene in Catla catla
| العنوان: | Identification, cDNA Cloning, and Characterization of Luteinizing Hormone Beta Subunit (lhb) Gene in Catla catla |
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| المؤلفون: | Rupam Sharma, Mohd Ashraf Rather, Irfan Ahmad Bhat |
| المصدر: | Animal Biotechnology. 27:148-156 |
| بيانات النشر: | Informa UK Limited, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Models, Molecular, 0106 biological sciences, 0301 basic medicine, Untranslated region, endocrine system, Cyprinidae, Bioengineering, Biology, 010603 evolutionary biology, 01 natural sciences, 03 medical and health sciences, Complementary DNA, Animals, Coding region, Tissue Distribution, Amino Acid Sequence, Cloning, Molecular, Peptide sequence, Gene, Phylogeny, Genetics, DNA, Luteinizing Hormone, beta Subunit, Sequence Analysis, DNA, biology.organism_classification, Molecular biology, Catla, Open reading frame, 030104 developmental biology, Gobiocypris rarus, Animal Science and Zoology, Sequence Alignment, Biotechnology |
| الوصف: | Reproductive hormones play a significant role in the gonadal development and gametogenesis process of animals. In the present study luteinizing hormone beta, (lhb) subunit gene was cloned and characterized from the brain of Catla catla. The lhb full-length of cDNA sequence is 629 bp which consists of 43bp 5'-UTR (untranslated region) 447bp, ORF(open reading frame) and 139 bp of 3'-UTR respectively. The coding region of lhb gene encoded a peptide of 148 amino acids. The coding sequence of lhb gene consist of a single N-linked glycosylation site (NET) and 12 cysteine knot residues. Phylogenetic analysis of C. catla Lhβ deduced amino acid sequence showed high similarity with Carassius auratus followed by Gobiocypris rarus. 3D structure Lhβ protein comprises of five β-sheets and six coils/loops. The qPCR results revealed lhb mRNA is mainly expressed in the pituitary, ovary while moderate expression was observed in brain and testis. To best our knowledge, this is the first report on the identification, molecular characterization and structural information regarding luteinizing hormone in Indian major carp. |
| تدمد: | 1532-2378 1049-5398 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f786beddc740b5206734c86d948a0266Test https://doi.org/10.1080/10495398.2016.1140055Test |
| رقم الانضمام: | edsair.doi.dedup.....f786beddc740b5206734c86d948a0266 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322378 10495398 |
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