Dolichol: A Component of the Cellular Antioxidant Machinery
| العنوان: | Dolichol: A Component of the Cellular Antioxidant Machinery |
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| المؤلفون: | I Parentini, Alessio Donati, Francesco Lenci, Ranieri Bizzarri, Ettore Bergamini, Antonella Sgarbossa, Gabriella Cavallini |
| المصدر: | Lipids 51 (2016): 477–486. doi:10.1007/s11745-016-4137-x info:cnr-pdr/source/autori:Cavallini G.; Sgarbossa A.; Parentini I.; Bizzarri R.; Donati A.; Lenci F.; Bergamini E./titolo:Dolichol: A Component of the Cellular Antioxidant Machinery/doi:10.1007%2Fs11745-016-4137-x/rivista:Lipids/anno:2016/pagina_da:477/pagina_a:486/intervallo_pagine:477–486/volume:51 |
| بيانات النشر: | Wiley, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Aging, Antioxidant, Ultraviolet Rays, medicine.medical_treatment, alpha-Tocopherol, medicine.disease_cause, Biochemistry, Antioxidants, Lipid peroxidation, 03 medical and health sciences, chemistry.chemical_compound, ?-Tocopherol, 0302 clinical medicine, Dolichol, Dolichols, medicine, TBARS, Animals, Cells, Cultured, chemistry.chemical_classification, Reactive oxygen species, Coenzyme Q, Phospholipid-bound polyunsaturated fatty acids, Rat hepatocytes, Oxidative stress, Catabolism, Organic Chemistry, food and beverages, Cell Biology, Rats, 030104 developmental biology, chemistry, Hepatocytes, lipids (amino acids, peptides, and proteins), Lipid Peroxidation, Mevalonate pathway, Reactive Oxygen Species, 030217 neurology & neurosurgery, Chromatography, Liquid |
| الوصف: | Dolichol, an end product of the mevalonate pathway, has been proposed as a biomarker of aging, but its biological role, not to mention its catabolism, has not been fully understood. UV-B radiation was used to induce oxidative stress in isolated rat hepatocytes by the collagenase method. Effects on dolichol, phospholipid-bound polyunsaturated fatty acids (PL-PUFA) and known lipid soluble antioxidants [coenzyme Q (CoQ) and ?-tocopherol] were studied. The increase in oxidative stress was detected by a probe sensitive to reactive oxygen species (ROS). Peroxidation of lipids was assessed by measuring the release of thiobarbituric acid reactive substances (TBARS). Dolichol, CoQ, and ?-tocopherol were assessed by high-pressure liquid chromatography (HPLC), PL-PUFA by gas-liquid chromatography (GC). UV-B radiation caused an immediate increase in ROS as well as lipid peroxidation and a simultaneous decrease in the levels of dolichol and lipid soluble antioxidants. Decrease in dolichol paralleled changes in CoQ levels and was smaller to that in ?-tocopherol. The addition of mevinolin, a competitive inhibitor of the enzyme 3-hydroxy-3-methylglutaryl CoA reductase (HMG-CoAR), magnified the loss of dolichol and was associated with an increase in TBARS production. Changes in PL-PUFA were minor. These findings highlight that oxidative stress has very early and similar effects on dolichol and lipid soluble antioxidants. Lower levels of dolichol are associated with enhanced peroxidation of lipids, which suggest that dolichol may have a protective role in the antioxidant machinery of cell membranes and perhaps be a key to understanding some adverse effects of statin therapy. |
| تدمد: | 1558-9307 0024-4201 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7026565424b8023333c32e8154f0649Test https://doi.org/10.1007/s11745-016-4137-xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f7026565424b8023333c32e8154f0649 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15589307 00244201 |
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