Effects of ChREBP deficiency on adrenal lipogenesis and steroidogenesis
| العنوان: | Effects of ChREBP deficiency on adrenal lipogenesis and steroidogenesis |
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| المؤلفون: | Yukio Horikawa, Ken Takao, Teruaki Sakurai, Sodai Kubota, Kenta Nonomura, Katsumi Iizuka, Saki Kubota-Okamoto, Daisuke Yabe, Yanyan Liu, Takehiro Kato, Toshinori Imaizumi, Yoshihiro Takahashi, Tetsuya Suwa, Masakatsu Sone, Tokuyuki Hirose, Yermek Rakhat, Satoko Komori, Masami Mizuno |
| المصدر: | Journal of Endocrinology. 248:317-324 |
| بيانات النشر: | Bioscientifica, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, 030209 endocrinology & metabolism, Adrenocorticotropic hormone, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Corticosterone, Internal medicine, Adrenal Glands, medicine, Animals, Carbohydrate-responsive element-binding protein, Fatty acid synthesis, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Adrenal gland, Cholesterol, Lipogenesis, Mice, Inbred C57BL, Steroid hormone, 030104 developmental biology, medicine.anatomical_structure, chemistry |
| الوصف: | Carbohydrate response element-binding protein (ChREBP) is critical in the regulation of fatty acid and triglyceride synthesis in the liver. Interestingly, Chrebp−/− mice show reduced levels of plasma cholesterol, which is critical for steroid hormone synthesis in adrenal glands. Furthermore, Chrebp mRNA expression was previously reported in human adrenal glands. Thus, it remains to be investigated whether ChREBP plays a role directly or indirectly in steroid hormone synthesis and release in adrenal glands. In the present study, we find that Chrebp mRNA is expressed in mouse adrenal glands and that ChREBP binds to carbohydrate response elements. Histological analysis of Chrebp−/− mice shows no adrenal hyperplasia and less oil red O staining compared with that in WT mice. In adrenal glands of Chrebp−/− mice, expression of Fasn and Scd1, two enzymes critical for fatty acid synthesis, was substantially lower and triglyceride content was reduced. Expression of Srebf2, a key transcription factor controlling synthesis and uptake of cholesterol and the target genes, was upregulated, while cholesterol content was not significantly altered in the adrenal glands of Chrebp−/− mice. Adrenal corticosterone content and plasma adrenocorticotropic hormone and corticosterone levels were not significantly altered in Chrebp−/− mice. Consistently, expression of genes related to steroid hormone synthesis was not altered. Corticosterone secretion in response to two different stimuli, namely 24-h starvation and cosyntropin administration, was also not altered in Chrebp−/− mice. Taking these results together, corticosterone synthesis and release were not affected in Chrebp−/− mice despite reduced plasma cholesterol levels. |
| تدمد: | 1479-6805 0022-0795 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6f6d4be6f01277e23edef96b850cc6bTest https://doi.org/10.1530/joe-20-0442Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f6f6d4be6f01277e23edef96b850cc6b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14796805 00220795 |
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