Clinical usefulness of fully automated chemiluminescent immunoassay for quantitative antibody measurements in COVID-19 patients
| العنوان: | Clinical usefulness of fully automated chemiluminescent immunoassay for quantitative antibody measurements in COVID-19 patients |
|---|---|
| المؤلفون: | Benoît Kabamba-Mukadi, Mehdi Khourssaji, Hector Rodriguez-Villalobos, Mathilde Berghmans, Jean Cyr Yombi, Reza Soleimani, Leila Belkhir, Damien Gruson |
| المساهمون: | UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, UCL - SSS/IREC/EDIN - Pôle d'endocrinologie, diabète et nutrition, UCL - SSS/IREC/LTAP - Louvain Centre for Toxicology and Applied Pharmacology, UCL - SSS/IREC/SLUC - Pôle St.-Luc, UCL - (SLuc) Service de biochimie médicale, UCL - (SLuc) Service de microbiologie, UCL - (SLuc) Service de médecine interne générale |
| المصدر: | Journal of medical virology, Vol. 93, no. 3, p. 1465-1477 (2021) Journal of Medical Virology |
| بيانات النشر: | Wiley-Liss, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, MaglumiTM, serology, Antibodies, Viral, Gastroenterology, SARS‐CoV‐2, Serology, 0302 clinical medicine, 030212 general & internal medicine, Research Articles, Aged, 80 and over, Immunoassay, biology, medicine.diagnostic_test, Reverse Transcriptase Polymerase Chain Reaction, MAGLUMI, Middle Aged, Infectious Diseases, Fully automated, Viral pneumonia, Female, 030211 gastroenterology & hepatology, Antibody, medicine.symptom, Research Article, Adult, medicine.medical_specialty, Adolescent, Coronavirus disease 2019 (COVID-19), Sensitivity and Specificity, Asymptomatic, COVID-19 Serological Testing, Young Adult, 03 medical and health sciences, COVID‐19, Internal medicine, Virology, medicine, Humans, immunoassay, Aged, business.industry, SARS-CoV-2, COVID-19, medicine.disease, Confidence interval, Immunoglobulin M, Immunoglobulin G, Luminescent Measurements, Euroimmun, biology.protein, business |
| الوصف: | Since December 2019, we have been in the battlefield with a new threat to the humanity, known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), characterized by viral pneumonia. It may be asymptomatic or cause various symptoms, ranging from flu-like symptoms to acute respiratory distress syndrome and eventually death. At present, the only reliable test for COVID-19 diagnosis is quantitative reverse transcriptase-polymerase chain reaction. Assessing the immune response against SARS-CoV-2 could increase the detection sensitivity of infected population. Hereby, we report the performances of a fully automated chemiluminescent immunoassay (CLIA) on 276 serum samples. One hundred samples obtained from COVID-19 negative subjects (COVID-19 free) were analyzed to evaluate the diagnostic specificity of antibody (Ab) detection. Thereafter, 176 samples obtained from 125 patients with confirmed COVID-19 (COVID-19 patients) were selected to assess the diagnostic sensitivity of the CLIA. All samples were analyzed on MAGLUMI 800 platform. All COVID-19 free samples had Ab levels below the cutoff values. Hence, the diagnostic specificity was estimated at 100% (95% confidence interval [CI] = 96.3-100.0; positive predictive value = 100%). By the 18th day from the onset of symptoms, we reached an optimal diagnostic sensitivity (more than 95.0%) In fact, the diagnostic sensitivity increased over time and between 15 and 25 days after symptoms onset, reached 95.5% (95% CI = 84.9-99.2). The new automated CLIA analyzer appeared to be a robust and reliable method to measure specific Ab against COVID-19 at high throughput. Our data suggest that combining Ab and nucleic acid detection could increase diagnostic sensitivity. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5ba64da99e912b5df157d3ff121d648Test https://hdl.handle.net/2078.1/243825Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f5ba64da99e912b5df157d3ff121d648 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |